A new high-sensitivity 10-ns time-resolution spectrophotometric technique adapted to in vivo analysis of the photosynthetic apparatus
A new high-sensitivity 10-ns time-resolution spectrophotometric technique adapted to in vivo analysis of the photosynthetic apparatus
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DOI：
10.1063/1.1149566
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发表时间：
1999-01-01
影响因子：
1.6
通讯作者：
Joliot, P
中科院分区：
文献类型：
--
作者：
Béal, D;Rappaport, F;Joliot, P
本文提出了一种新的分光光度法，即利用Nd：YAG激光三次谐波泵浦的光参量振荡器（OPO）产生的短闪光对吸收进行取样。连续可调的单色闪光（6纳秒的持续时间，1纳米的带宽），获得的域范围从415至680 nm和730至2000 nm。光诱导的吸收变化可以在10 ns至100 s的时间窗口内进行分析，噪声水平为10（-5）。这项新技术开辟了以前现有的温度计无法进入的研究领域。事实上，探测闪光的高能量允许在光谱的蓝色区域分析大光密度的样品，例如树叶。应用这种方法的几个例子，在不同类型的光合机构（叶，单细胞藻类和细菌）的电子转移反应的分析。该技术也非常适合于分析溶液或活细胞中血红素蛋白相关配体的光解离。(C)1999年美国物理学会。[S0034-6748（99）02501-0]。
A new spectrophotometric technique has been developed in which the absorption is sampled by short flashes produced by an optical parametric oscillator (OPO) pumped by the third harmonic of a Nd: YAG laser. Continuously tunable monochromatic flashes (6 ns duration, 1 nm bandwidth) are obtained in a domain ranging from 415 to 680 nm and 730 to 2000 nm. Light-induced absorption changes can be analyzed in a time window ranging from 10 ns to 100 s, with a 10(-5) noise level. This new technique opens areas of research which are not accessible to previously existing spectrophotometers. Indeed, the high energy of the detecting flashes allows the analysis of samples of large optical density such as leaves, in the blue region of the spectrum. A few examples of application of this method to the analysis of electron transfer reactions in different types of photosynthetic apparatus (leaves, unicellular algae, and bacteria) are presented. This technique is also well suited to analyze the photodissociation of ligands associated with hemoproteins, in solution or in living cells. (C) 1999 American Institute of Physics. [S0034-6748(99)02501-0].