Immunohistochemical analysis of malignant melanomas and nevocellular nevi with monoclonal antibodies to distinct monomorphic determinants of HLA antigens.
Immunohistochemical analysis of malignant melanomas and nevocellular nevi with monoclonal antibodies to distinct monomorphic determinants of HLA antigens.
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发表时间：
1984-09
期刊：
影响因子：
11.2
通讯作者：
D. J. Ruiter;Wilma Bergman;Kees Welvaart;Erik Scheffer;W. A. Vloten;Carlo Russo;S. Ferrane
中科院分区：
文献类型：
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作者：
D. J. Ruiter;Wilma Bergman;Kees Welvaart;Erik Scheffer;W. A. Vloten;Carlo Russo;S. Ferrane
使用间接免疫过氧化物酶技术，20痣细胞痣，5发育不良痣，14原发性皮肤黑色素瘤，和24转移性黑色素瘤进行了测试与一组单克隆抗体单态决定簇的I类（HLA-A，B，C）和II类（Ia样）主要组织相容性复合物抗原。I类HLA和β 2-微球蛋白在大多数痣细胞上未检测到，但在5个发育不良痣中有3个表达，在14个原发性皮肤黑色素瘤中有12个表达，在24个转移瘤中有13个表达。Ⅰ类HLA和β 2-微球蛋白在原发灶和转移灶中的不同表达提示这些抗原的丢失可能与恶性肿瘤的进展有关。II类HLA在普通痣中未检测到，但在5例发育不良痣中的1例、14例原发性皮肤黑色素瘤中的7例和所有24例转移性病变中局部存在。这些结果表明，增加II类HLA表达可能与恶性肿瘤的进展。用单克隆抗体对不同决定簇的I类HLA和II类HLA获得的染色模式在每种类型的抗原内是可重叠的。因此，文献中关于黑素细胞源性病变组织相容性抗原表达的差异不太可能反映不同研究者使用的抗体的不同特异性。
Using an indirect immunoperoxidase technique, 20 nevocellular nevi, 5 dysplastic nevi, 14 primary cutaneous melanomas, and 24 metastatic melanomas were tested with a panel of monoclonal antibodies to monomorphic determinants of Class I (HLA-A,B,C) and Class II (la-like) major histocompatibility complex antigens. Class I HLA and beta 2-microglobulins were not detected on the majority of nevus cells but were expressed by 3 of 5 dysplastic nevi, by the majority of tumor cells in 12 of 14 primary cutaneous melanomas, and in 13 of 24 metastases. The different expression of Class I HLA and beta 2-microglobulins in primary and metastatic lesions suggests that loss of these antigens may be associated with progression of malignancy. Class II HLA were not detected in common nevi but were locally present in 1 of 5 dysplastic nevi, 7 of 14 cases of primary cutaneous melanoma, and all 24 cases of metastatic lesions tested. These findings suggest that increase in Class II HLA expression may be associated with progression of malignancy. The staining patterns obtained with monoclonal antibodies to distinct determinants of Class I HLA and Class II HLA were superimposable within each type of antigen. Therefore, the discrepancies in the literature about the expression of histocompatibility antigens by lesions of melanocytic origin are not likely to reflect the different specificity of the antibodies used by the various investigators.