Correlation of higher antibody levels to pneumococcal proteins with protection from pneumococcal acute otitis media but not protection from nasopharyngeal colonization in young children.
Correlation of higher antibody levels to pneumococcal proteins with protection from pneumococcal acute otitis media but not protection from nasopharyngeal colonization in young children.
复制标题
较高的肺炎球菌蛋白抗体水平与预防肺炎球菌急性中耳炎相关，但与预防幼儿鼻咽定植无关。
DOI：
10.1016/j.cmi.2017.01.011
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Pichichero,ME
中科院分区：
文献类型：
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作者：
Xu,Q;Casey,JR;Almudevar,A;Pichichero,ME
目的我们以前发现，鼻咽（NP）定植肺炎链球菌eliminate粘膜抗体应答三种蛋白疫苗候选：肺炎球菌组氨酸三联体蛋白D（PhtD），肺炎球菌胆碱结合蛋白A（PcpA），和解毒肺炎球菌溶血素（PlyD 1）。在这里，我们试图确定是否粘膜抗体水平的蛋白质与保护急性中耳炎（AOM）和NP colonization.MethodsA共228 NP样本前瞻性收集100名健康婴儿在6-24个月的年龄。每当儿童被诊断为AOM时，收集中耳液以通过微生物培养来确认诊断。NP粘膜IgG和伊加定量ELISA.ResultsHigher NP mucosal antibody levels to S. pneumoniae proteins correlated with significantly decreased possibility of developing AOM caused by S. pneumoniaeduring 3 to 12 months of subsequent prospective monitoring.具体来说，没有经历AOM的儿童（n= 111份样本）的粘膜IgG水平比PcpA高2 - 5倍（所有p值<0.01），伊加比PhtD高6 - 8倍（所有p值<0.05）;伊加比PcpA高两到三倍（所有p值<0.05），与发生AOM的儿童（n= 18个样本）相比，PlyD 1的伊加高2 - 3倍（p 0.08，p 0.03和p 0.08）。结论NP粘膜IgG对PcpA、伊加对PhtD、PcpA和PlyD 1的抗体水平升高与肺炎链球菌AOM感染的发生风险降低相关，但与NP定植风险降低无关。
ObjectivesWe previously found that nasopharyngeal (NP) colonization byStreptococcus pneumoniaeelicits mucosal antibody responses to three protein vaccine candidates: pneumococcal histidine triad protein D (PhtD), pneumococcal choline-binding protein A (PcpA), and detoxified pneumolysin (PlyD1). Here we sought to determine if mucosal antibody levels to the proteins correlated with protection from acute otitis media (AOM) and NP colonization.MethodsA total of 228 NP samples were prospectively collected from 100 healthy infants at 6–24 months of age. Whenever children were diagnosed with AOM, middle ear fluids were collected to confirm the diagnosis by microbiological culture. NP mucosal IgG and IgA were quantified by ELISA.ResultsHigher NP mucosal antibody levels toS. pneumoniaeproteins correlated with significantly decreased likelihood of developing AOM caused byS. pneumoniaeduring 3 to 12 months of subsequent prospective monitoring. Specifically, children who did not experience AOM (n= 111 samples) caused byS. pneumoniaehad two- to five-fold higher mucosal IgG levels to PcpA (all p values <0.01), six- to eight-fold higher IgA to PhtD (all p values <0.05); two- to three-folder higher IgA to PcpA (all p values <0.05), and two- to three-fold higher IgA to PlyD1 (p 0.08, p 0.03 and p 0.08) compared with children who did experience AOM (n= 18 samples). No association between mucosal antibody levels to the three proteins and NP colonization withS. pneumoniaewas found.ConclusionHigher NP mucosal IgG levels to PcpA, and IgA to PhtD, PcpA and PlyD1 correlate with reduced risk of development ofS. pneumoniaeAOM infection but not with reduced risk of NP colonization in young children.