Numerical determination of transmembrane voltage induced on irregularly shaped cells
Numerical determination of transmembrane voltage induced on irregularly shaped cells
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DOI：
10.1007/s10439-005-9076-2
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发表时间：
2006-04-01
影响因子：
3.8
通讯作者：
Miklavcic, D
中科院分区：
文献类型：
--
作者：
Pucihar, G;Kotnik, T;Miklavcic, D
本文提出了一种方法，减少了与在不规则形状细胞上测定诱导跨膜电压（ITV）有关的几个困难。我们首先描述了一种基于显微成像构建不规则形状细胞的现实模型的方法。这提供了在进行实验的相同细胞上确定ITV的可能性，并且对于理解和解释数据具有相当重要的意义。我们还展示了如何用一个边界条件取代有限厚度、非零电导率的膜，在这个边界条件下，细胞内部（细胞质）和外部之间的界面具有特定的表面电导率。通过与孤立球形电池和倾斜扁球形电池的解析解进行比较，验证了该方法的结果，得到了很好的一致性。此外，我们比较了两个不规则形状的CHO细胞模型计算的ITV与通过电位荧光染料在相同的两个细胞上测量的ITV，以及这两个细胞的简化模型计算的ITV。
The paper presents an approach that reduces several difficulties related to the determination of induced transmembrane voltage (ITV) on irregularly shaped cells. We first describe a method for constructing realistic models of irregularly shaped cells based on microscopic imaging. This provides a possibility to determine the ITV on the same cells on which an experiment is carried out, and can be of considerable importance in understanding and interpretation of the data. We also show how the finite-thickness, nonzero-conductivity membrane can be replaced by a boundary condition in which a specific surface conductivity is assigned to the interface between the cell interior (the cytoplasm) and the exterior. We verify the results obtained using this method by a comparison with the analytical solution for an isolated spherical cell and a tilted oblate spheroidal cell, obtaining a very good agreement in both cases. In addition, we compare the ITV computed for a model of two irregularly shaped CHO cells with the ITV measured on the same two cells by means of a potentiometric fluorescent dye, and also with the ITV computed for a simplified model of these two cells.