Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences
Comparison of ancient and modern Clonorchis sinensis based on ITS1 and ITS2 sequences
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DOI：
10.1016/j.actatropica.2006.08.010
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发表时间：
2007-02-01
期刊：
影响因子：
2.7
通讯作者：
Li, Yong-Long
中科院分区：
文献类型：
--
作者：
Liu, Wen-Qi;Liu, Juan;Li, Yong-Long
1975年，在中国湖北省江陵县发现一具埋藏于公元前167年的古尸。尸体胆囊中发现的华支睾吸虫卵保存完好。在本文中，我们分别从古代鸡蛋和现代鸡蛋中提取基因组DNA，并对核糖体RNA基因的内转录间隔区1和2（ITS1和ITS2）进行研究。结果表明，古代样品的ITS2序列与现代样品的序列相同，但在ITS I中，发现古代样品和现代样品之间有15个核苷酸位置存在差异。结果表明，从古代寄生虫卵中提取 DNA 并进行测序是可能的。获得的 ITS I 序列与迄今为止可用的所有现代序列不同。这可能表明序列随时间的分歧，或者可能反映最终可能在现代样本中发现的序列多态性。 (c) 2007 年 Elsevier B.V. 出版
In 1975, an ancient corpse buried in 167 BC was found at Jiangling County, Hubei Province of China. The eggs of Clonorchis sinensis found in the gall bladder of the corpse were preserved well. In the present paper, we extracted the genomic DNA from the ancient eggs and modern eggs, respectively, and the internal transcribed spacer 1 and 2 (ITS1 and ITS2) at ribosomal RNA genes were studied. The results show that ITS2 sequences from the ancient sample were identical with those from modern samples, but in ITS I differences in 15 nucleotide positions were found between the ancient and modern samples. The results demonstrated that it is possible to extract and sequence DNA from ancient parasite eggs. The ITS I sequence obtained differed from all modern ones available to date. This might indicate sequence divergence through time, or might reflect a sequence polymorphism that may eventually be found also in modern samples. (c) 2007 Published by Elsevier B.V.