Dual-Functional Polyethylene Glycol-b-polyhexanide Surface Coating with in Vitro and in Vivo Antimicrobial and Antifouling Activities
Dual-Functional Polyethylene Glycol-b-polyhexanide Surface Coating with in Vitro and in Vivo Antimicrobial and Antifouling Activities
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具有体外和体内抗菌和防污活性的双功能聚乙二醇-b-聚己缩酰胺表面涂层
DOI：
10.1021/acsami.6b12979
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发表时间：
2017
影响因子：
9.5
通讯作者：
Huang Wei
中科院分区：
文献类型：
--
作者：
Zhi Zelun;Su Yajuan;Xi Yuewei;Tian Liang;Xu Miao;Wang Qianqian;Padidan Sara;Li Peng;Huang Wei
近年来，生物医学植入物/器械表面的微生物定植已成为严重威胁人类健康的问题。在此，表面固定化胍衍生物嵌段共聚物创建了一种抗菌和防污双功能涂层。我们报道了用聚己烷（PHMB）与烯丙基缩水甘油酯醚或烯丙氧基聚乙二醇（APEG；分子量1200和2400）偶联制备一种抗菌防污嵌段共聚物。采用等离子体-紫外辅助表面引发聚合的方法，将烯丙基甘油酯醚改性PHMB （a- PHMB）和烯丙基聚乙二醇1200/2400改性PHMB （APEG1200/2400-PHMB）共聚物分别接枝到硅橡胶表面，形成瓶刷状涂层。A-PHMB和APEG1200/2400-PHMB涂层对革兰氏阴性/阳性细菌和真菌均表现出优异的广谱抗菌性能。与其他两种涂层相比，APEG2400-PHMB涂层具有更好的抗菌膜、防污性能和更长的可重复使用周期，这是由于其共聚物中含有丰富的PEG嵌段。此外，apeg2400 - phmb涂层硅胶片与哺乳动物细胞具有生物相容性，这是体外血液相容性和细胞毒性试验的结果。一项体内研究显示，大肠杆菌数量明显下降，减少了5倍，表明APEG2400-PHMB涂层表面在啮齿动物皮下感染模型中有效。这种基于phmb的嵌段共聚物涂层被认为是预防生物材料相关感染的有效策略。
In recent years, microbial colonization on the surface of biomedical implants/devices has become a severe threat to human health. Herein, surface-immobilized guanidine derivative block copolymers create an antimicrobial and antifouling dual-functional coating. We report the preparation of an antimicrobial and antifouling block copolymer by the conjugation of polyhexanide (PHMB) with either allyl glycidyl ether or allyloxy polyethylene glycol (APEG; MW 1200 and 2400). The allyl glycidyl ether modified PHMB (A-PHMB) and allyloxy polyethylene glycol1200/2400modified PHMB (APEG1200/2400-PHMB) copolymers were grafted onto a silicone rubber surface as a bottlebrush-like coating, respectively, using a plasma-UV-assisted surface-initiated polymerization. Both A-PHMB and APEG1200/2400-PHMB coatings exhibited excellent broad-spectrum antimicrobial properties against Gram-negative/positive bacteria and fungi. The APEG2400-PHMB coating displayed an improved antibiofilm as well as antifouling properties and a long reusable cycle, compared with two other coatings, due to its abundant PEG blocks among those copolymers. Also, the APEG2400-PHMB-coated silicone coupons were biocompatible toward mammalian cells, as revealed by in vitro hemocompatibile and cytotoxic assays. An in vivo study showed a significant decline ofEscherichia colicolonies with a 5-log reduction, indicating the APEG2400-PHMB coating surface worked effectively in the rodent subcutaneous infection model. This PHMB-based block copolymer coating is believed to be an effective strategy to prevent biomaterial-associated infections.