Genome Sequence of the Chestnut Blight Fungus Cryphonectria parasitica EP155: A Fundamental Resource for an Archetypical Invasive Plant Pathogen
Genome Sequence of the Chestnut Blight Fungus Cryphonectria parasitica EP155: A Fundamental Resource for an Archetypical Invasive Plant Pathogen
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DOI：
10.1094/phyto-12-19-0478-a
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发表时间：
2020-06-01
期刊：
影响因子：
3.2
通讯作者：
Nuss, Donald L.
中科院分区：
文献类型：
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作者：
Crouch, Jo Anne;Dawe, Angus;Nuss, Donald L.
栗疫病是栗疫病的病原体，栗疫病是一种真菌疾病，在50年的时间里几乎完全消除了北美成熟的美国栗。在这里，我们正式报告了C。寄生菌EP 155的基因组序列，使用桑格鸟枪测序方法进行测序。在完成并整合简单序列重复标记后，在26个支架中的组装体为43.8Mb（L-50 = 5; N-50 = 4.0Mb）。预测八条染色体：五个支架具有两个端粒，六个支架具有一个端粒序列。总共预测了11，609个基因模型，其中85%与其他蛋白质相似。该基因组资源已经通过对真菌营养体不亲和性系统的新理解增加了基本植物病原体实验系统的实用性，对增强基于真菌病毒的生物防治具有重要意义。
Cryphonectria parasitica is the causal agent of chestnut blight, a fungal disease that almost entirely eliminated mature American chestnut from North America over a 50-year period. Here, we formally report the genome of C. parasitica EP155 using a Sanger shotgun sequencing approach. After finishing and integration with simple-sequence repeat markers, the assembly was 43.8 Mb in 26 scaffolds (L-50 = 5; N-50 = 4.0Mb). Eight chromosomes are predicted: five scaffolds have two telomeres and six scaffolds have one telomere sequence. In total, 11,609 gene models were predicted, of which 85% show similarities to other proteins. This genome resource has already increased the utility of a fundamental plant pathogen experimental system through new understanding of the fungal vegetative incompatibility system, with significant implications for enhancing mycovirus-based biological control.