Characteristics of humic substances in the Kuji River waters as determined by high-performance size exclusion chromatography with fluorescence detection.
Characteristics of humic substances in the Kuji River waters as determined by high-performance size exclusion chromatography with fluorescence detection.
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DOI：
10.1016/s0043-1354(03)00377-4
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发表时间：
2003-10
期刊：
影响因子：
12.8
通讯作者：
S. Nagao;T. Matsunaga;Yasuhiro Suzuki;T. Ueno;H. Amano
中科院分区：
文献类型：
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作者：
S. Nagao;T. Matsunaga;Yasuhiro Suzuki;T. Ueno;H. Amano
采用高效体积排阻色谱结合荧光检测技术，对流经日本森林丘陵和农业平原的久治河水系河水中的腐殖质进行了直接测定。监测波长为激发波长320 nm，发射波长430 nm，与水生黄腐酸的最大荧光波长相对应。河水的色谱图显示有四个峰，每个峰的位置在这些样品之间很好地吻合。库集河上游及其支流的样品峰高比与库集河中下游不同，这可能反映了土壤向河流提供的腐殖质和其他有机物的特征不同。
Direct measurement by high-performance size exclusion chromatography with fluorescence detection was applied to the characterization of humic substances in river waters from the Kuji River system, which runs through forest hills and an agricultural plain in Japan. The monitoring wavelength of excitation 320nm and emission 430nm corresponds to the fluorescence maxima for aquatic fulvic acid. Chromatograms of the river waters showed four peaks; each peak position was in good agreement among these samples. Peak height ratios for the samples from the upstream Kuji River and its tributaries were different from those of the midstream and downstream sections of the Kuji River, which may reflect differences in the characteristics of humic substances and other organic materials supplied from soil to river.