Information theoretical approach to single-molecule experimental design and interpretation.
Information theoretical approach to single-molecule experimental design and interpretation.
复制标题
单分子实验设计和解释的信息理论方法。
DOI：
10.1021/jp062192b
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Talaga,DavidS
中科院分区：
文献类型：
--
作者：
Talaga,DavidS
我们使用香农的信息定义来发展一个理论来预测基于光子计数的单分子实验测量所需属性的能力。我们对待三种现象，通常测量单分子：光谱波动的溶剂化显色染料;分配的方位角偶极角;确定的距离荧光共振能量转移使用福斯特的理论。我们考虑了背景和其他“不完美”的测量通过减少信息的影响。
We use Shannon's definition of information to develop a theory to predict a photon-counting-based single-molecule experiment's ability to measure the desired property. We treat three phenomena that are commonly measured on single molecules: spectral fluctuations of a solvatochromic dye; assignment of the azimuthal dipole angle; determination of a distance by fluorescence resonant energy transfer using Förster's theory. We consider the effect of background and other “imperfections” on the measurement through the decrease in information.