Description of 'Candidatus Helicobacter heilmannii' based on DNA sequence analysis of 16S rRNA and urease genes
Description of 'Candidatus Helicobacter heilmannii' based on DNA sequence analysis of 16S rRNA and urease genes
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DOI：
10.1099/ijs.0.63117-0
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发表时间：
2004-11-01
影响因子：
2.8
通讯作者：
Lee, A
中科院分区：
文献类型：
--
作者：
O'Rourke, JL;Solnick, JV;Lee, A
虽然幽门螺杆菌被认为是人类胃部疾病的主要细菌因子，但一些患者和许多动物感染了以前称为“Helicobacter heilmannii”或“Gastroplellum hominis”的较大的紧密螺旋形细菌。这些细菌的分类学分类一直受到阻碍，无法培养他们在体外和16S rRNA基因序列分析的歧视权力不足。本研究描述了26种不同的人和动物的胃肠道菌分离株的检测和系统发育分析，其中包括基于16S rRNA和尿素酶基因的序列数据。在人类、灵长类动物和猪中检测到的15种胃肠道菌与“猪幽门螺杆菌”聚集在一起，从而扩大了这种生物的宿主范围。通过比较，基于16S rRNA数据，其余11个腹足类。不能与猫螺杆菌、bizzozeronii螺杆菌和Salomonis螺杆菌鉴别。然而，尿素酶基因序列分析允许歧视后一组成四个离散的集群，其中三个包含上述公认的物种。第四簇包含来自人类和猫科动物宿主的分离株，暂时应被认为是一种独特的细菌物种，建议将其命名为“Helicobacter heilmannii”。
While Helicobacter pylori is accepted as the major bacterial agent of gastric disease in humans, some patients and many animals are infected with a larger, tightly helical-shaped bacterium previously referred to as 'Helicobacter heilmannii' or 'Gastrospirillum hominis'. Taxonomic classification of these bacteria has been hampered by the inability to cultivate them in vitro and by the inadequate discriminatory power of 16S rRNA gene sequence analysis. This study describes the detection and phylogenetic analysis of 26 different gastrospirillum isolates from humans and animals, which incorporates sequence data based on the 16S rRNA and urease genes. Fifteen gastrospirilla detected in humans, primates and pigs clustered with 'Candidatus Helicobacter suis', thus expanding the host range for this organism. By comparison, based on 16S rRNA data, the remaining 11 gastrospirilla. could not be differentiated from Helicobacter felis, Helicobacter bizzozeronii and Helicobacter salomonis. However, urease gene sequence analysis allowed for the discrimination of this latter group into four discrete clusters, three of which contained the above recognized species. The fourth cluster contained isolates from human and feline hosts, and should provisionally be considered a unique bacterial species, for which the name 'Candidatus Helicobacter heilmannii' is proposed.