Initial T cell receptor transgenic cell precursor frequency dictates critical aspects of the CD8+ T cell response to infection
Initial T cell receptor transgenic cell precursor frequency dictates critical aspects of the CD8+ T cell response to infection
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DOI：
10.1016/j.immuni.2007.04.013
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发表时间：
2007-06-01
期刊：
影响因子：
32.4
通讯作者：
Harty, John T.
中科院分区：
文献类型：
--
作者：
Badovinac, Vladimir P.;Haring, Jodie S.;Harty, John T.
Adoptive-transfer experiments with relatively large input numbers (similar to 10(6)) of T cell receptor-transgenic (TCR-tg) T cells are widely used to model endogenous T cell responses to infection or immunization. We show that input numbers of naive TCR-tg T cells sufficient to squelch the endogenous response to the same epitope substantially alter the kinetics, proliferative expansion, phenotype, and efficiency of memory generation by the TCR-tg T cells in response to infection. Thus, responses from nonphysiologic input numbers of TCR-tg T cells fail to accurately mimic the endogenous T cell response. Importantly, seeding as few as similar to 10-50 TCR-tg T cells, which constitute a fraction of the endogenous repertoire, allowed vigorous proliferation and analysis of TCR-tg cells after infection in a scenario representing normal physiology for any individual TCR. These data strongly suggest that modeling the endogenous T cell response with TCR-tg cells will require every effort to approximate the endogenous precursor frequency.
