Light-driven CO2 sequestration in Escherichia coli to achieve theoretical yield of chemicals
Light-driven CO2 sequestration in Escherichia coli to achieve theoretical yield of chemicals
复制标题
光驱动大肠杆菌中的CO2封存以实现化学品的理论产量
DOI：
10.1038/s41929-021-00606-0
复制
发表时间：
2021-04-29
期刊：
影响因子：
37.8
通讯作者：
Chen, Xiulai
中科院分区：
文献类型：
--
作者：
Hu, Guipeng;Li, Zehong;Chen, Xiulai
提高异养微生物中的碳产率的效率是生物制造所期望的。目前，一种与产品无关的、节能的CO2封存系统已经被开发出来，该系统可以最大限度地提高碳转化率，化学品的生产达到其理论产量。CO2封存工程是实现碳高效和节能的生物生产的一种有吸引力的策略。然而，异养CO2封存的效率受到生物产品依赖和能量不足的限制。在这里，开发了模块化CO2封存工程，通过集成合成CO2固定和CO2减排模块来生产目标化学品。设计了一种合成的CO2固定途径，然后使用自组装硫化镉纳米颗粒通过光驱动还原功率来增强。接下来，设计了一个CO2缓解开关，然后通过Proteorhodopsin通过光驱动能量进行优化。最后，通过整合CO2固定和CO2缓解模块，CO2封存的效率显着提高大肠杆菌和l-苹果酸和丁酸的产量分别增加到1.48和0.79 mol/mol葡萄糖，达到理论产量。这种二氧化碳封存系统为将二氧化碳转化为增值化学品提供了一个有效的平台。
Improving the efficiency of carbon yield in heterotrophic microorganisms is desired for biomanufacturing. Now, a product-independent and energy-efficient CO2 sequestration system that maximizes carbon conversion has been developed, as showcased by the production of chemicals reaching their theoretical yields.CO2 sequestration engineering is an attractive strategy for achieving carbon- and energy-efficient bioproduction. However, the efficiency of heterotrophic CO2 sequestration is limited by bioproduct dependence and energy deficiency. Here, modular CO2 sequestration engineering was developed to produce target chemicals by integrating synthetic CO2 fixation and CO2 mitigation modules. A synthetic CO2 fixation pathway was designed, and then enhanced by light-driven reducing power using self-assembled cadmium sulfide nanoparticles. Next, a CO2 mitigation switch was designed, and then optimized by light-driven energy via proteorhodopsin. Finally, by integrating CO2 fixation and CO2 mitigation modules, the efficiency of CO2 sequestration was notably enhanced in Escherichia coli and the yields of l-malate and butyrate were increased to 1.48 and 0.79 mol/mol glucose, respectively, reaching theoretical yields. This CO2 sequestration system provides an efficient platform for channelling CO2 into value-added chemicals.