Comparison of methods for detection of Erysipelothrix spp. and their distribution in some Australasian seafoods
Comparison of methods for detection of Erysipelothrix spp. and their distribution in some Australasian seafoods
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DOI：
10.1128/aem.66.5.2066-2070.2000
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发表时间：
2000-05-01
影响因子：
4.4
通讯作者：
Riley, TV
中科院分区：
文献类型：
--
作者：
Fidalgo, SG;Wang, Q;Riley, TV
多年来，丹毒丝菌rhusiopathiae已被认为是职业相关感染丹毒的病原体。丹毒丝菌的分布调查。在19个澳大利亚的海产品进行，丹毒丝菌属的检测方法。进行了评估。21株丹毒丝菌从52份海产品中分离到10株大肠杆菌。丹毒丝菌属的初步分离，最有效地实现了脑心输液肉汤富集，继代培养到选择性脑心输液琼脂含有卡那霉素，新霉素，万古霉素孵育48小时后。选择性胰蛋白酶大豆肉汤培养48 h是检测丹毒丝菌的最佳培养方法。用PCR。PCR检测的敏感性比培养高50%。E. rhusiopathiae分离自多种不同的鱼类、头足类动物和甲壳类动物，包括西部岩龙虾（Panuliruscygnss）。海产品产地与大肠杆菌的分布没有明显的相关性。红斑病从一只澳大利亚牡蛎和一只银鲷中首次分离到一种与扁桃体丹毒丝菌难以区分的微生物。总的来说，丹毒丝菌属（丹毒丝菌属）广泛分布在澳大利亚的海产品，说明丹毒样感染的渔民的潜力。
For many years, Erysipelothrix rhusiopathiae has been known to be the causative agent of the occupationally related infection erysipeloid. A survey of the distribution of Erysipelothrix spp. in 19 Australasian seafoods was conducted, and methodologies for the detection of Erysipelothrix spp. were evaluated. Twenty-one Erysipelothrix spp. were isolated from 52 seafood parts. Primary isolation of Erysipelothrix spp, was most efficiently achieved with brain heart infusion broth enrichment followed by subculture onto a selective brain heart infusion agar containing kanamycin, neomycin, and vancomycin after 48 h of incubation. Selective tryptic soy broth, with 48 h of incubation, was the best culture method for the detection of Erysipelothrix spp. with PCR. PCR detection was 50% more sensitive than culture. E. rhusiopathiae was isolated from a variety of different fish, cephalopods, and crustaceans, including a Western rock lobster (Panulirus cygnss). There was no significant correlation between the origin of the seafoods tested and the distribution of E. rhusiopathiae. An organism indistinguishable from Erysipelothrix tonsillarum was isolated for the first time from an Australian oyster and a silver bream. Overall, Erysipelothrix spp. were widely distributed in Australasian seafoods, illustrating the potential for erysipeloid-like infections in fishermen.