Dynamic spatial reorganization of BSK1 complexes in the plasma membrane underpins signal-specific activation for growth and immunity
Dynamic spatial reorganization of BSK1 complexes in the plasma membrane underpins signal-specific activation for growth and immunity
复制标题
质膜中 BSK1 复合物的动态空间重组支撑生长和免疫的信号特异性激活
DOI：
10.1016/j.molp.2021.01.019
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发表时间：
2021-04-05
期刊：
影响因子：
27.5
通讯作者：
Lin, Jinxing
中科院分区：
文献类型：
--
作者：
Su, Bodan;Zhang, Xi;Lin, Jinxing
生长和免疫是竞争细胞资源的对立过程，适当的资源分配对植物的生存至关重要。BSK1分别与BRI1和FLS2结合，在生长和免疫调控中发挥关键作用。然而，目前尚不清楚两种拮抗信号如何协同BSK1诱导信号特异性激活。本研究表明，BSK1在质膜内的动态空间重组是信号特异性激活生长或免疫机制的基础。静止的BSK1以复合物的形式定位于膜筏。与BSK1相关的FLS2和BRI1不同，flg22或外源性油菜素类固醇（BR）处理并未降低质膜（PM）上的BSK1水平，而是分别诱导BSK1与FLS2/BSK1或BRI1/BSK1聚合和分离。此外，flg22激活的BSK1从膜筏转移到非膜筏区域，而br激活的BSK1留在膜筏中。当与flg22一起使用时，BR抑制了flg22诱导的BSK1的各种活性，如BSK1与FLS2/BSK1的分离，BSK1与MAPKKK5的相互作用，以及BSK与MAPKKK5的易位。综上所述，本研究为BSK1时空组织的精确控制如何调节信号特异性以平衡植物生长和免疫提供了独特的见解。
Growth and immunity are opposing processes that compete for cellular resources, and proper resource allocation is crucial for plant survival. BSK1 plays a key role in the regulation of both growth and immunity by associating with BRI1 and FLS2, respectively. However, it remains unclear how two antagonistic signals co-opt BSK1 to induce signal-specific activation. Here we show that the dynamic spatial reorganization of BSK1 within the plasma membrane underlies the mechanism of signal-specific activation for growth or immunity. Resting BSK1 localizes to membrane rafts as complexes. Unlike BSK1-associated FLS2 and BRI1, flg22 or exogenous brassinosteroid (BR) treatment did not decrease BSK1 levels at the plasma membrane (PM) but rather induced BSK1 multimerization and dissociation from FLS2/BSK1 or BRI1/BSK1, respectively. Moreover, flg22-activated BSK1 translocated from membrane rafts to non-membrane-raft regions, whereas BR-activated BSK1 remained in membrane rafts. When applied together with flg22, BR suppressed various flg22-induced BSK1 activities such as BSK1 dissociation from FLS2/BSK1, BSK1 interaction with MAPKKK5, and BSK translocation together with MAPKKK5. Taken together, this study provides a unique insight into how the precise control of BSK1 spatiotemporal organization regulates the signaling specificity to balance plant growth and immunity.