Temperature-dependent sex differentiation in goldfish: Establishing the temperature-sensitive period and effect of constant and fluctuating water temperatures
Temperature-dependent sex differentiation in goldfish: Establishing the temperature-sensitive period and effect of constant and fluctuating water temperatures
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DOI：
10.1016/j.aquaculture.2005.10.009
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发表时间：
2006-04-28
期刊：
影响因子：
4.5
通讯作者：
Yamauchi, K
中科院分区：
文献类型：
--
作者：
Goto-Kazeto, R;Abe, Y;Yamauchi, K
本研究分析了具有XX-XY性别决定系统的金鱼的温度依赖性性别分化。在23℃+/-0.5℃饲养至第12天(受精后)，将XX雄×××杂交后代分为15℃、17℃、20℃、23℃、25℃、28℃、30℃+-0.5℃饲养，雌体比例分别为：15℃94.6%、17℃100%、20℃94.6%、23℃46.6%、28℃15.4%，在30℃和0.5℃条件下，雌虫的表型雌体表型转化为雄体表型。为了确定性别分化的温度敏感期(TSP)，测量了随着发育进程在23-30℃之间倒置的幼虫组的性别比。在第17天、第22天和第27天，试验组雌性动物的百分比从23摄氏度增加到30摄氏度，而在第17天和22天，雌性动物的百分比从30摄氏度下降到23摄氏度。TSP的终点出现在第17天30摄氏度和第22天23摄氏度，这意味着TSP在更高的温度下缩短。为了研究温度波动对性分化的影响，在TSP过程中，施加了从23到30摄氏度或30到23摄氏度的几个一天的温度脉冲。从30摄氏度的基础温度到23摄氏度的脉冲导致雄性比例下降，然而，从23摄氏度的基础温度到30摄氏度的脉冲对性别比例没有影响。这些结果表明，在TSP过程中必须保持高温，才能产生高比例的雄性遗传雌性金鱼。总之，水温可以在金鱼的性别分化过程中发挥重要作用，就像在其他硬骨鱼和爬行动物中看到的那样，并可作为控制性别的工具应用于水产养殖。(C)2005 Elsevier B.V.保留所有权利。
The present study analyzes the temperature-dependent sex differentiation in goldfish, which have an XX-XY sex determining system. Hatchlings from an XX male-XX female cross were reared at 23 +/- 0.5 degrees C until Day 12 (days after fertilization), and divided into experimental groups reared at 15, 17, 20, 23, 25, 28 or 30 +/- 0.5 degrees C. The proportions of females were 94.6% at 15 degrees C, 100% at 17 degrees C, 94.6% at 20 degrees C 90.5% at 23 degrees C 46.6% at 25 degrees C, 15.4% at 28 degrees C, and 7.7% at 30 0.5 degrees C. This result confirms that genotypic females can be changed to phenotypic males by high temperature treatment. To determine the temperature sensitive period (TSP) of sex differentiation, the sex ratio was measured in groups of larvae shifted reciprocally between 23 and 30 degrees C as development progressed. The percentage of females increased in test groups shifted from 23 to 30 degrees C at Days 17, 22 and 27 and decreased in groups shifted from 30 to 23 degrees C at Day 17 and 22. The end point of the TSP occurs on Day 17 at 30 degrees C and on Day 22 at 23 degrees C, implying that the TSP is shortened at higher temperatures. To investigate the effect of fluctuating temperatures on sex differentiation, several one-day temperature pulses from 23 to 30 degrees C or 30 to 23 degrees C were applied, during the TSP. The pulse to 23 degrees C, from the basal temperature of 30 degrees C, resulted in a decreased percentage of males, however, the pulse to 30 degrees C, from the basal temperature of 23 degrees C, had no effect on the sex ratio. Theses results show that high temperature must be maintained during the TSP in order to produce a high proportion of masculinized genetic female goldfish. In conclusion, water temperature can play an important role in the process of sex differentiation in goldfish, as has been seen in other teleosts and reptiles, and be applied to aquaculture as a tool for control of sex. (c) 2005 Elsevier B.V. All rights reserved.