Inhibition of DNA replication and growth of several human and murine neoplastic cells by aphidicolin without detectable effect upon synthesis of immunoglobulins and HLA antigens.
Inhibition of DNA replication and growth of several human and murine neoplastic cells by aphidicolin without detectable effect upon synthesis of immunoglobulins and HLA antigens.
复制标题
阿菲迪霉素抑制 DNA 复制和几种人和鼠肿瘤细胞的生长，对免疫球蛋白和 HLA 抗原的合成没有可检测到的影响。
作者：
G. Pedrali;M. Belvedere;T. Crepaldi;F. Focher;S. Spadari
Aphidicolin 抑制所有测试的人和鼠肿瘤细胞的 DNA 复制和生长，包括白血病 T 淋巴细胞和 B 淋巴细胞以及黑色癌细胞。在所有测试的肿瘤细胞系中引起 DNA 合成抑制 50% 的阿菲迪霉素浓度与在 HeLa 细胞中抑制 DNA 合成 50% 所需的浓度相似。抑制肿瘤细胞中 DNA 合成的机制同样是由于阿菲迪霉素对 DNA 聚合酶 α 的抑制。比引起DNA合成和细胞生长抑制50%的浓度高100倍的阿菲迪霉素对总蛋白合成、免疫球蛋白的分泌或参与免疫反应相关现象的HLA抗原的表达没有影响。
Aphidicolin inhibits DNA replication and growth of all tested human and murine neoplastic cells including leukemic T- and B-lymphocytes and melanocarcinoma cells. The concentration of aphidicolin causing 50% inhibition of DNA synthesis in all of the tested neoplastic cell lines is similar to that necessary to inhibit DNA synthesis in HeLa cells by 50%. The mechanism of inhibition of DNA synthesis in neoplastic cells is again due to the inhibition of DNA polymerase alpha by aphidicolin. Aphidicolin at a concentration 100 times higher than that causing 50% inhibition of DNA synthesis and cell growth had no effect on total protein synthesis, on the secretion of immunoglobulins, or on the expression of HLA antigens which are involved in relevant phenomena of the immune response.