A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP
A simple and rapid method for isolation of high quality genomic DNA from fruit trees and conifers using PVP
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DOI：
10.1093/nar/25.5.1085
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发表时间：
1997-03-01
影响因子：
14.9
通讯作者：
Hyung, NI
中科院分区：
文献类型：
--
作者：
Kim, CS;Lee, CH;Hyung, NI
由于DNA降解是由次生植物产物如酚萜类介导的，因此从含有高含量多酚类物质的植物中分离出高质量的DNA一直是一个难题。我们通过修改几个现有的提取过程来演示一个简单的提取过程。利用这种方法，我们发现可以在几个小时内从葡萄（Vitis spp.）、苹果（Malus spp.）、梨（Pyrus spp.）和柿子（Diospyros spp.）四种果树以及松柏（Pinus densiflora）、红松（Pinus koraiensis）、东北红豆杉（Taxus cuspidata）和中国桧（Juniperus chinensis）四种树种中分离dna。与现有方法相比，我们分离到了高质量的完整DNA(260/280 = 1.8-2.0)，常规产量为250-500 ng/ μ l（从4 -5个组织盘中提取的DNA总量为7.5-15 μ g）。
Because DNA degradation is mediated by secondary plant products such as phenolic terpenoids, the isolation of high quality DNA from plants containing a high content of polyphenolics has been a difficult problem. We demonstrate an easy extraction process by modifying several existing ones. Using this process we have found it possible to isolate DNAs from four fruit trees, grape (Vitis spp.), apple (Malus spp.), pear (Pyrus spp,) and persimmon (Diospyros spp.) and four species of conifer, Pinus densiflora, Pinus koraiensis, Taxus cuspidata and Juniperus chinensis within a few hours. Compared with the existing method, we have isolated high quality intact DNAs (260/280 = 1.8-2.0) routinely yielding 250-500 ng/mu l (total 7.5-15 mu g DNA from four to five tissue discs).