Coordinate expression of nuclear and mitochondrial genes involved in energy production in carcinoma and oncocytoma
Coordinate expression of nuclear and mitochondrial genes involved in energy production in carcinoma and oncocytoma
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DOI：
10.1016/0925-4439(96)00026-9
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发表时间：
1996-08-23
影响因子：
6.2
通讯作者：
Stepien, G
中科院分区：
文献类型：
--
作者：
Heddi, A;FaureVigny, H;Stepien, G
在肾癌、肾嗜酸细胞瘤和唾液嗜酸细胞瘤中检查了参与 ATP 产生的线粒体和核基因的表达。研究发现肾癌的线粒体 DNA (mtDNA) 含量减少，而嗜酸细胞瘤的 mtDNA 含量增加。这与这些肿瘤中线粒体数量的形态变化相似。在癌症中，mtDNA 转录物相对于对照肾脏减少了 5 至 10 倍，这表明线粒体转录物水平取决于 mtDNA 含量。在肾嗜酸细胞瘤中，尽管 mtDNA 含量较高，但 mtDNA 转录物略有减少。然而，在唾液腺嗜酸细胞瘤中，mtDNA 转录物增加了 10 倍以上，同时 mtDNA 含量也增加了 10 倍。肾癌中核 DNA 氧化磷酸化基因 ATPsyn beta 和 ANT2 的表达降低达 4 倍。相比之下，这两种核基因转录物的水平在肾嗜酸细胞瘤中被诱导约4倍，在唾液腺嗜酸细胞瘤中高达30倍。此外，观察到 ANT2 前体在嗜酸细胞瘤中发生变化。这些数据表明肾癌中核和线粒体基因表达的协调调节以及嗜酸细胞瘤中核 OXPHOS 基因表达的特异性诱导。
The expression of mitochondrial and nuclear genes involved in ATP production was examined in renal carcinomas, renal oncocytomas, and a salivary oncocytoma. Renal carcinomas were found to have a reduced mitochondrial DNA (mtDNA) content while oncocytomas had increased mtDNA contents. This parallels morphological changes in mitochondrial number in these tumours. In the carcinomas, mtDNA transcripts were decreased 5- to 10-fold relative to control kidneys, suggesting that mitochondrial transcript levels depend on the mtDNA content. In renal oncocytomas, mtDNA transcripts were slightly reduced in spite of a high mtDNA content. However, in the salivary gland oncocytoma, mtDNA transcripts were increased more than 10-fold in parallel with a 10-fold increase in mtDNA content. The expression of the nuclear DNA oxidative phosphorylation genes, ATPsyn beta and ANT2, was reduced up to 4-fold in renal carcinoma. In contrast, the levels of these two nuclear gene transcripts were induced about 4-fold in renal oncocytoma and up to 30-fold in salivary gland oncocytoma. Moreover, the ANT2 precursors were observed to change in oncocytomas. These data suggest a coordinated regulation of nuclear and mitochondrial gene expression in renal carcinomas and the specific induction of nuclear OXPHOS gene expression in oncocytomas.