THE CHARACTERISTICS REQUIRED FOR A SENDAI VIRUS PREPARATION TO INDUCE HIGH-LEVELS OF INTERFERON IN HUMAN-LYMPHOBLASTOID CELLS
THE CHARACTERISTICS REQUIRED FOR A SENDAI VIRUS PREPARATION TO INDUCE HIGH-LEVELS OF INTERFERON IN HUMAN-LYMPHOBLASTOID CELLS
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DOI：
10.1099/0022-1317-56-1-175
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发表时间：
1981-01-01
影响因子：
3.8
通讯作者：
JOHNSTON, MD
中科院分区：
文献类型：
--
作者：
JOHNSTON, MD
鸡蛋培养的仙台病毒制剂在人淋巴母细胞Namalwa中诱导干扰素的能力取决于其传代史。以高稀释度连续传代的病毒是不良诱导剂;未稀释连续传代的病毒是良好诱导剂。由于高含量的缺陷干扰（DI）颗粒，这种良好的诱导剂制剂具有低的感染性与血凝比率。使用在甘油梯度上纯化的DI颗粒，结果表明，对于最大干扰素滴度的诱导，需要感染性和DI颗粒。单独的DI颗粒诱导很少或不诱导干扰素。向完全感染性仙台病毒制剂中加入DI颗粒可使从Namalwa细胞获得的干扰素产量增加约60至100倍。
The capacity of an egg-grown Sendai virus preparation to induce interferon in the human lymphoblastoid cell Namalwa is dependent on its passage history. Virus which was serially passaged at high dilution is a poor inducer; virus serially passaged undiluted is a good inducer. Such a good inducer preparation has a low infectivity to hemagglutination ratio as the result of a high content of defective-interfering (DI) particles. Using DI particles purified on glycerol gradients, it is shown that for the induction of maximum interferon titers both infectious and DI particles are required. DI particles alone induce little or no interferon. Addition of DI particles to fully infectious Sendai virus preparations increased the interferon yield obtained from Namalwa cells some 60- to 100-fold.