Modulation by general anaesthetics of rat GABA(A) receptors comprised of alpha 1 beta 3 and beta 3 subunits expressed in human embryonic kidney 293 cells
Modulation by general anaesthetics of rat GABA(A) receptors comprised of alpha 1 beta 3 and beta 3 subunits expressed in human embryonic kidney 293 cells
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DOI：
10.1038/sj.bjp.0700987
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发表时间：
1997-03-01
影响因子：
7.3
通讯作者：
Hales, TG
中科院分区：
文献类型：
--
作者：
Davies, PA;Kirkness, EF;Hales, TG
1 使用放射性配体结合和膜片钳技术研究 γ-氨基丁酸 (GABA) 和全身麻醉药丙泊酚（2,6-二异丙基苯酚）、戊巴比妥和 5 α-pregnan-3 α-ol-20-one 对单独或组合表达的大鼠 α 1 和 β 3 GABA(A) 受体亚基的作用。2 HEK293 细胞在用 alpha 1 cDNA 转染不会结合显着水平的 [S-35]-叔丁基二环硫代磷酸酯 ([S-35]-TBPS)（
1 Radioligand binding and patch-clamp techniques were used to study the actions of gamma-aminobutyric acid (GABA) and the general anaesthetics propofol (2,6-diisopropylphenol), pentobarbitone and 5 alpha-pregnan-3 alpha-ol-20-one on rat alpha 1 and beta 3 GABA(A) receptor subunits, expressed either alone or in combination.2 Membranes from HEK293 cells after transfection with alpha 1 cDNA did not bind significant levels of [S-35]-tert-butyl bicyclophosphorothionate ([S-35]-TBPS) (