Plastome evolution and phylogenomics of Impatiens (Balsaminaceae)
Plastome evolution and phylogenomics of Impatiens (Balsaminaceae)
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DOI：
10.1007/s00425-023-04078-3
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发表时间：
2023-02-01
期刊：
影响因子：
4.3
通讯作者：
Li，Pan
中科院分区：
文献类型：
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作者：
Qiu，He;Zhang，Zhi-Hui;Li，Pan
主要结论：本研究报道了7个来自impatiens的新质体，并观察到3个系统发育和DNA条形码高度可变的区域，解决了impatiens亚属各区段之间的关系。香茅科（Balsaminaceae, Ericales）是被子植物中最大和最多样化的属之一，因其分类困难而广为人知。在这项研究中，我们使用完整的质体序列数据重新评估了凤仙花属内的遗传关系。对凤仙花（impatiens）的7个完整质体体（代表6种）与其他凤仙花属（Hydrocera triflora, Balsaminaceae;Marcgravia coriacea, marcgraaviaceae）的20个质体体进行了新测序和鉴定。29个质体体的总大小在151538 ~ 152917 bp之间，但凤仙花（impatiens morsei）的2个质体体长度较短，且缺失部分编码NADH脱氢酶亚基的基因。简单重复序列（SSRs）在51 ~ 113个之间，长重复序列在17 ~ 26个之间。此外，还鉴定出三个高度可变的区域（trnG-GCC（前一个），ndhF-rpl32-trnL-UGA-ccsA, andycf1）。我们基于80个质体衍生的蛋白质编码基因的系统基因组分析有力地支持了impatiens及其两个亚属（clavicarpaan和dimpatiens）的单系性，并完全解决了impatiens亚属中6个（7个）样本片段之间的关系。总之，本研究报道的质体DNA标记和系统发育结果将为今后凤仙花的鉴定、分类和DNA条形码研究以及凤仙花科植物的进化研究提供参考。
Main conclusionThis study reported seven new plastomes fromImpatiensand observed three highly variable regions for phylogeny and DNA barcoding, which resolved the relationships among sections of subgenusImpatiens.AbstractImpatiensL. (Balsaminaceae, Ericales) is one of the largest and most diverse genera of angiosperms, widely known for its taxonomic difficulty. In this study, we reevaluated the infrageneric relationships within the genusImpatiens, using complete plastome sequence data. Seven complete plastomes ofImpatiens(representing 6 species) were newly sequenced and characterized along with 20 previously published plastomes of otherImpatiensspecies, plus 2 plastomes of outgroups (Hydrocera triflora, Balsaminaceae;Marcgravia coriacea, Marcgraviaceae). The total size of these 29 plastomes ranged from 151,538 bp to 152,917 bp, except 2 samples ofImpatiens morsei, which exhibited a shorter length and lost some genes encoding NADH dehydrogenase subunits. Moreover, the number of simple sequence repeats (SSRs) ranged from 51 to 113, and the number of long repeats from 17 to 26. In addition, three highly variable regions were identified (trnG-GCC(The previous one),ndhF–rpl32–trnL-UGA–ccsA, andycf1). Our phylogenomic analysis based on 80 plastome-derived protein-coding genes strongly supported the monophyly ofImpatiensand its two subgenera (ClavicarpaandImpatiens), and fully resolved relationships among the six (out of seven) sampled sections of subgenusImpatiens. Overall, the plastome DNA markers and phylogenetic results reported in this study will facilitate future identification, taxonomic and DNA barcoding studies inImpatiensas well as evolutionary studies in Balsaminaceae.