Coupling Reaction-Based Ultrasensitive Detection of Phenolic Estrogens Using Surface-Enhanced Resonance Raman Scattering
Coupling Reaction-Based Ultrasensitive Detection of Phenolic Estrogens Using Surface-Enhanced Resonance Raman Scattering
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DOI：
10.1021/ac2019766
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发表时间：
2011-11-15
影响因子：
7.4
通讯作者：
Ozaki, Yukihiro
中科院分区：
文献类型：
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作者：
Han, Xiao Xia;Pienpinijtham, Prompong;Ozaki, Yukihiro
研究表明，许多不利的健康影响与人类接触饮食或环境雌激素有关。因此，发展快速、高灵敏的雌激素检测方法对于维持激素浓度在安全范围内是非常重要和必要的。在这里，我们展示了一种简单而快速的方法来检测痕量的酚类雌激素基于表面增强共振拉曼散射(SERRS)。由于重氮离子与酚类雌激素发生偶联反应，形成具有很强的SerRS活性的偶氮化合物，从而使溶液中的酚类雌激素能够在亚纳摩尔水平上识别。由于偶氮染料的每个SerRS指纹特定地对应于相关的雌激素，因此所提出的协议具有多路复用能力。此外，它具有普适性和高度选择性，不仅对酚类雌激素，而且对其他酚类分子，甚至在复杂的体系中也是如此。
Studies have shown that many adverse health effects are associated with human exposure to dietary or environmental estrogens. Therefore, the development of rapid and highly sensitive detection methods for estrogens is very important and necessary to maintain hormonal concentration below the safety limit. Herein, we demonstrate a simple and rapid approach to detect trace amounts of phenolic estrogen based on surface-enhanced resonance Raman scattering (SERRS). Because of a coupling reaction between diazonium ions and the phenolic estrogens, azo compounds are formed with strong SERRS activity, which allows phenolic estrogen recognition at subnanomolar levels in solution. The proposed protocol has multiplexing capability, because each SERRS fingerprint of the azo dyes specifically corresponds to the related estrogen. Moreover, it is universal and highly selective, not only for phenolic estrogens but also for other phenolic molecules, even in complex systems.