Systemic and exhaled cytokine and chemokine profiles are associated with the development of bronchiolitis obliterans syndrome
Systemic and exhaled cytokine and chemokine profiles are associated with the development of bronchiolitis obliterans syndrome
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DOI：
10.1016/j.healun.2010.04.013
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发表时间：
2010-09-01
影响因子：
8.9
通讯作者：
Van Den Bosch, Jules M. M.
中科院分区：
文献类型：
--
作者：
Kastelijn, Elisabeth A.;Rijkers, Ger T.;Van Den Bosch, Jules M. M.
背景技术背景：提示T辅助细胞（Th）1和Th 2细胞因子的相互作用可能是导致闭塞性细支气管炎综合征（BUS）纤维化闭塞的机制之一。本研究的目的是确定肺移植受者血清和呼出气冷凝液（EBC）中的Th I和Th 2细胞因子和趋化因子谱，并评估其作为预测BUS发展的生物标志物的有用性。来自10名BUS患者（BOSPOS）和10名无BOS患者（BOSneg）的血清和EBC，匹配临床和人口统计学变量，结果：肺移植受者血清中促炎细胞因子水平较对照组升高。与BOSneg患者相比，BOSpos患者的白细胞介素（IL）-4，IL-13和血管内皮生长因子（VEGF）浓度显著降低。然而，BOSpos患者的IL-5浓度显著较高。EBC中IL-4和IL-5水平几乎检测不到。与BOSneg患者相比，BOSpos患者血清中的IL-13和VEGF均降低，BOSpos患者EBC中的IL-13和VEGF也降低。纵向分析的细胞因子和趋化因子的血清和EBC从肺移植的时间起没有发现显着的趋势，细胞因子和趋化因子的诊断之前BOS.CONCLUSIONS：促炎细胞因子的水平增加肺移植受者与对照组相比。从移植开始，BOSpos患者血清中Th 2细胞因子的模式与BOSneg患者不同。J Heart Lung Transplant 2010;29：997-1008（C）2010年国际心肺移植学会。All rights reserved.
BACKGROUND: The mechanisms that lead to the fibrotic obliteration in bronchiolitis obliterans syndrome (BUS) may involve the interactions between T-helper (Th)1 and Th2 cytokines. The aim of this study is to determine the Th I and Th2 cytokine and chemokine profiles in serum and exhaled breath condensate (EBC) in lung transplant recipients and to assess their usefulness as biomarkers to predict the development of BUS.METHODS: Serum and EBC from 10 patients with BUS (BOSPOS) and 10 patients without BOS (BOSneg), matched for clinical and demographic variables, were analyzed with a multiplex immunoassay to measure a panel of 27 cytokines and chemokines.RESULTS: The pro-inflammatory cytokines in serum were elevated in lung transplant recipients compared with controls. BOSpos patients had significantly lower concentrations of interleukin (IL)-4, IL-13, and vascular endothelial growth factor (VEGF) compared with BOSneg patients. The concentration of IL-5, however, was significantly higher in BOSpos patients. Levels of IL-4 and IL-5 were hardly detectable in EBC. IL-13 and VEGF, both decreased in serum in BOSpos patients, were also decreased in EBC in BOSpos patients compared with BOSneg patients. Longitudinal analysis of cytokines and chemokines in serum and EBC from the time of lung transplantation onwards did not reveal significant trends in cytokines and chemokines that preceded the diagnosis of BOS.CONCLUSIONS: Levels of pro-inflammatory cytokines were increased in lung transplant recipients compared with controls. From the moment of transplantation onwards, there is a different pattern of Th2 cytokines in serum in BOSpos patients than in BOSneg patients. J Heart Lung Transplant 2010;29:997-1008 (C) 2010 International Society for Heart and Lung Transplantation. All rights reserved.