Cyclic Tripeptides from the Halotolerant Fungus Aspergillus sclerotiorum PT06-1
Cyclic Tripeptides from the Halotolerant Fungus Aspergillus sclerotiorum PT06-1
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DOI：
10.1021/np100198h
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发表时间：
2010-06-01
影响因子：
5.1
通讯作者：
Zhu, Weiming
中科院分区：
文献类型：
--
作者：
Zheng, Jinkai;Xu, Zhihong;Zhu, Weiming
从耐盐菌株Aspergillus sclerotiorum PT 06 -1发酵液的乙酸乙酯提取物中分离得到11个新的aspochracin型环三肽，即sclerotiotides A-K（1-11），以及3个已知化合物JBIR-15（12）、aspochracin（13）和青霉酸。通过波谱分析和化学方法鉴定了它们的结构。12和13的化学转化证明sclerotiotides D K（4-11）可能是在发酵或随后的分离步骤中形成的人工产物。所有13个环三肽已被评估其抗菌和细胞毒性作用。只有sclerotiotitides A（1）、13（2）、F（6）和I（9）和JBIR-15（12）对白色念珠菌显示选择性抗真菌活性，MIC值分别为7.5、3.8、30、6.7和30 μ M。
Eleven new aspochracin-type cyclic tripeptides, sclerotiotides A-K (1-11), together with three known compounds, JBIR-15 (12), aspochracin (13), and penicillic acid, were isolated from the ethyl acetate extract of the fermentation broth of the halotolerant Aspergillus sclerotiorum PT06-1 in a hypersaline nutrient-rich medium. Their structures were elucidated by spectroscopic analysis and chemical methods. Chemical transformations of 12 and 13 proved that sclerotiotides D K (4-11) were artifacts probably formed during the fermentation or subsequent isolation steps. All 13 cyclic tripeptides have been evaluated for their antimicrobial and cytotoxic effects. Only sclerotiotides A (1), 13 (2), F (6), and I (9) and JBIR-15 (12) showed selective antifungal activity against Candida albicans with MIC values of 7.5, 3.8, 30, 6.7, and 30 mu M, respectively.