Hyperglycosylation of glycopeptidolipid of Mycobacterium smegmatis under nutrient starvation:: structural studies
Hyperglycosylation of glycopeptidolipid of Mycobacterium smegmatis under nutrient starvation:: structural studies
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DOI：
10.1099/mic.0.27908-0
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发表时间：
2005-07-01
期刊：
影响因子：
2.8
通讯作者：
Chatterji, D
中科院分区：
文献类型：
--
作者：
Mukherjee, R;Gomez, M;Chatterji, D
糖肽脂（GPL）的极性物种在碳饥饿的耻垢分枝杆菌中的存在以前已经报道过。在这项研究中，该GPL的完整结构是建立与MALDI-TOF（基质辅助激光解吸/电离飞行时间）和ESI（电喷雾电离）-MS，C-13-SEFT（自旋回波傅里叶变换）-MVR光谱，和HPLC分析的帮助。在该分子中，两个单元的鼠李糖的3，4-二-O-甲基衍生物通过1 -> 2键连接到L-丙氨醇上。合成了鼠李糖和6-脱氧塔罗糖的各种甲基衍生物作为标准品以建立该结构。这种极性GPL在M.作为SigB高产菌株，丝霉毒素是sigB依赖性的。smeglycin显示在指数期存在该斑点，而sigB敲除的M. Smeglavin没有显示任何极性GPL的存在。
The presence of a polar species of glycopepticlolipid (GPL) in carbon-starved Mycobacterium smegmatis has been reported previously. In this study, the complete structure of this GPL is established with the help of MALDI-TOF (matrix assisted laser desorption/ionization time of flight) and ESI (electrospray ionization) -MS, C-13-SEFT (spin echo Fourier transform) -MVR spectroscopy, and HPLC analysis. In the molecule, two units of a 3,4-di-O-methyl derivative of rhamnose are attached to L-alaninol via a 1 -> 2 linkage. Various methyl derivatives of rhamnose and 6-deoxytalose were synthesized as standards to establish this structure. The accumulation of this polar GPL in M. smegmatis is sigB dependent, as a SigB-overproducing strain of M. smegmatis shows the presence of this spot in the exponential phase, and a sigB-knockout strain of M. smegmatis does not show the presence of any polar GPLs.