Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa
Global transcriptome profiling analysis reveals insight into saliva-responsive genes in alfalfa
复制标题
全球转录组分析揭示了苜蓿唾液响应基因的见解
DOI：
10.1007/s00299-015-1903-9
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发表时间：
2016-03-01
影响因子：
6.2
通讯作者：
Liu, Zhipeng
中科院分区：
文献类型：
--
作者：
Liu, Wenxian;Zhang, Zhengshe;Liu, Zhipeng
Saliva deposition is one of the key factors influencing plant-herbivore interactions during grazing. Although many studies have focused on the effects of saliva deposition on plant regrowth, no consistent conclusions have been reached. Alfalfa is the most extensively cultivated forage legume, yet most alfalfa cultivars, thus far, are not grazing-tolerant. To better understand the underlying mechanism, we undertook a study to evaluate the global changes in the transcriptome of alfalfa after cow saliva deposition treatment. In this study, cDNA libraries from alfalfa seedlings at 0, 4, 8, and 24 h after cow saliva deposition were constructed and sequenced, resulting in the identification of 53,195 annotated unigenes, from which 4,814 unigenes were significantly differentially expressed. A metabolic pathway enrichment analysis demonstrated that saliva deposition functions as a stress that negatively affects the regrowth of alfalfa by modifying jasmonic acid synthesis, enhancing the susceptibility to pathogens and reducing the expression levels of ribosomal protein genes. In the present study, we demonstrate the potential effects of saliva deposition on alfalfa regrowth at the transcriptome level. These fundamental and important findings could facilitate further investigations into the molecular mechanisms underlying the responses of alfalfa and other related species to herbivore grazing.
