TURNIP CRINKLE VIRUS GENES REQUIRED FOR RNA REPLICATION AND VIRUS MOVEMENT
TURNIP CRINKLE VIRUS GENES REQUIRED FOR RNA REPLICATION AND VIRUS MOVEMENT
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DOI：
10.1016/0042-6822(92)90055-t
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发表时间：
1992-01-01
期刊：
影响因子：
3.7
通讯作者：
MORRIS, TJ
中科院分区：
文献类型：
--
作者：
HACKER, DL;PETTY, ITD;MORRIS, TJ
我们已经用诱变芜菁皱缩病毒（TCV）cDNA克隆的感染性体外转录本来鉴定病毒RNA复制、病毒体组装和细胞间运动所需的基因产物。TCV基因组的先前序列分析揭示了五个开放阅读框的存在，其具有编码88、38、28、9和8kDa的基因产物的潜力。用感染性RNA接种原生质体显示，只有p28和p88基因产物是病毒RNA合成所需的。虽然p8和p9基因产物在原生质体中的RNA复制和病毒体组装是不稳定的，但p8和p9基因的突变阻止了植物中系统性感染的产生。没有病毒RNA或蛋白质中观察到的接种或系统的叶片接种与转录合成的p8或p9突变体cDNA的植物。与这些结果相反，病毒RNA从接种的，但不是系统的叶，接种的RNA缺乏外壳蛋白（CP）基因的植物中回收。CP突变体在正常系统性宿主上没有观察到症状，但在Chenopodium amaranticolor上诱导了小的局部病变。这些结果表明，p8、p9和CP是病毒移动所必需的。
We have used infectiousin vitrotranscripts from mutagenized turnip crinkle virus (TCV) cDNA clones to identify the gene products required for viral RNA replication, virion assembly, and intercellular movement. Previous sequence analysis of the TCV genome revealed the presence of five open reading frames which had the potential to encode gene products of 88, 38, 28, 9, and 8 kDa. Inoculation of protoplasts with infectious RNA revealed that only the p28 and p88 gene products are required for viral RNA synthesis. Although the p8 and p9 gene products were dispensable for RNA replication and virion assembly in protoplasts, mutations in the p8 and p9 genes prevented the production of systemic infections in plants. No viral RNA or protein was observed in the inoculated or systemic leaves of plants inoculated with transcripts synthesized from p8 or p9 mutant cDNAs. In contrast to these results, viral RNA was recovered from the inoculated, but not the systemic leaves, of plants inoculated with an RNA lacking the coat protein (CP) gene. With the CP mutant, no symptoms were observed on normally systemic hosts, but small local lesions were induced onChenopodium amaranticolor. These results indicate that p8, p9, and CP are required for viral movement.