Arabidopsis COG6 is essential for pollen tube growth and Golgi structure maintenance
Arabidopsis COG6 is essential for pollen tube growth and Golgi structure maintenance
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拟南芥 COG6 对于花粉管生长和高尔基体结构维持至关重要
DOI：
10.1016/j.bbrc.2020.05.189
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发表时间：
2020
影响因子：
3.1
通讯作者：
Bao Y.
中科院分区：
文献类型：
--
作者：
Rui Q.;Wang J.;Li Y.;Tan X.;Bao Y.
保守的寡聚体高尔基体（COG）复合体由8个亚基组成，命名为COG 1-COG 8，与大多数真核生物中存在的同源亚基高度保守。在酵母和哺乳动物中，COG复合物与逆行高尔基体内囊泡的束缚有关。虽然已经在拟南芥中鉴定了COG亚基的同源物，但复合物及其亚基的功能仍有待充分阐明。在这项研究中，我们利用遗传学和细胞学的方法来表征的COG 6亚基的作用。我们发现，COG 6突变导致雄性传递缺陷，由于异常的花粉管生长。在亚细胞水平上，高尔基体的形态发生了改变，导致花粉和细胞壁成分在花粉管中的沉积不正确。与绿色荧光蛋白（GFP）融合的COG 6定位于高尔基体，它补充了COG 6花粉管的异常生长。我们建议，COG 6，作为一个亚基的COG复合物，调节高尔基体形态和囊泡运输的稳态在花粉管生长。
The conserved oligomeric Golgi (COG) complex, which consists of eight subunits named COG1–COG8, is highly conserved with homologous subunits present in most eukaryotic species. In yeast and mammalian, the COG complex has been implicated in the tethering of retrograde intra-Golgi vesicles. Although homologs of COG subunits have been identified in Arabidopsis, the functions of the complex and its subunits remain to be fully elucidated. In this study, we have utilized genetic and cytologic approaches to characterize the role of the COG6 subunit. We showed that a mutation inCOG6caused male transmission defect due to aberrant pollen tube growth. At the subcellular level, Golgi bodies exhibited altered morphology incog6pollen and cell wall components were incorrectly deposited in pollen tubes. COG6 fused to green fluorescent protein (GFP), which complemented the aberrant growth ofcog6pollen tubes, was localized to the Golgi apparatus. We propose that COG6, as a subunit of the COG complex, modulates Golgi morphology and vesicle trafficking homeostasis during pollen tube growth.