Genetic and antigenic diversity of human caliciviruses (HuCVs) using RT-PCR and new EIAs.
Genetic and antigenic diversity of human caliciviruses (HuCVs) using RT-PCR and new EIAs.
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DOI：
10.1007/978-3-7091-6553-9_27
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Xi Jiang;D. Matson;W. Cubitt;M. Estes
中科院分区：
文献类型：
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作者：
Xi Jiang;D. Matson;W. Cubitt;M. Estes
使用杯状病毒基因组保守区的引物进行RT-PCR，然后进行测序，可以表征该家族内的遗传变异。建立了以杆状病毒表达的病毒衣壳蛋白和针对衣壳蛋白的高免抗血清为基础的EIA，用于检测人疱疹病毒抗原和抗体。利用重组诺瓦克病毒(RNV)和重组墨西哥病毒(RMX，一种类似SMA的病毒)进行的血清学调查表明，由HuCVs感染是常见的，在发达国家和发展中国家，儿童在很小的时候就获得了HuCVs的抗体。已经描述了以诺沃克病毒(NV)、雪山病毒(SMA)和札幌病毒为代表的三个HuCV基因组，尽管最近积累的HuCV毒株序列表明这些基因组可以进一步划分。根据重组EIA的结果，这些基因组还对应于不同的抗原组。这三个基因群共同循环并在世界范围内分布，尽管SMA基因群目前似乎占主导地位。应用这些新的检测方法来进一步表征HuCV的遗传和抗原性仍然是HuCV研究的重要任务。
RT-PCR using primers from conserved regions of calicivirus genomes, followed by sequencing, permits characterization of genetic variation within the family. EIAs based on baculovirus-expressed viral capsid proteins and hyperimmune antisera against the capsid proteins were developed to detect HuCV antigens and antibodies. Serologic surveys using recombinant Norwalk virus (rNV) and recombinant Mexico virus (rMX, a SMA-like virus) EIAs showed that infections by HuCVs are common and that children acquire antibodies to HuCVs at an early age in both developed and developing countries. Three HuCV genogroups have been described that are represented by Norwalk virus (NV), Snow Mountain agent (SMA), and Sapporo virus, although recently accumulated sequences of HuCV strains indicate these genogroups can be further divided. These genogroups also correspond to distinct antigenic groups based on the results of the recombinant EIAs. The three genogroups co-circulate and have a worldwide distribution, although the SMA genogroup seems to be predominant currently. Application of these new assays for further characterization of the genetic and antigenic properties of HuCVs remains an important task for HuCV research.