Altered Blood Flow Distribution in the Rat Spinal Cord under Chronic Compression
Altered Blood Flow Distribution in the Rat Spinal Cord under Chronic Compression
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DOI：
10.1097/brs.0b013e3181eaf33d
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发表时间：
2011-06-01
期刊：
影响因子：
3
通讯作者：
Kim, Phyo
中科院分区：
文献类型：
--
作者：
Kurokawa, Ryu;Murata, Hidetoshi;Kim, Phyo
研究设计.假手术对照动物研究，以评估慢性压迫模型中脊髓血流的变化。实验室调查。脊髓型颈椎病是老年患者致残的常见原因。提出脊髓受压后缺血在脊髓功能障碍的发病机制中起重要作用的假说。本研究旨在评估大鼠模型在慢性压迫下脊髓血流的变化。背景资料概要。脊髓型颈椎病患者的脊髓组织学研究显示缺血性组织改变。在实验上，脊髓低灌注结合慢性脊髓压迫诱导脊髓病的狗。我们以前表明，慢性压迫大鼠脊髓产生逐渐恶化的活动性的动物伴随着脊髓组织变性与缺血性变化兼容。通过在C5-C6椎板下植入薄的聚氨酯聚合物片来实施颈脊髓的慢性压迫，该聚氨酯聚合物片通过吸收组织水在48-72小时内膨胀。对照组行假手术。12周后，采用荧光微球法检测C3-C4和C5-C6脊髓节段的血流量。对照组C5-C6节段血流大于C3-C4节段。压迫组C5-C6节段血流明显低于C3-C4节段。在慢性局灶性脊髓压迫下，与喙段相比，压迫段的血流量减少。我们的数据与脊髓血流量的改变有助于脊髓病发病机制的假设是一致的。
Study Design. Sham-operation-controlled animal study to assess alterations in blood flow in the spinal cord in a chronic compression model. Laboratory investigation.Objective. Cervical myelopathy is a common cause of disability in elderly patients. Hypothesis was made that ischemia subsequent to the spinal cord compression plays an important role in the pathogenesis of the spinal cord dysfunction. This study was undertaken to assess alterations in the blood flow of the spinal cord under chronic compression in a rat model.Summary of Background Data. Histologic study of spinal cord from patients with spondylotic myelopathy showed ischemic tissue changes. Experimentally, spinal cord hypoperfusion in combination with chronic spinal cord compression induced myelopathy in dogs. We previously showed that chronic compression of the spinal cord in rats produces gradual deterioration of mobility of the animals accompanied by cord tissue degeneration compatible with ischemic changes.Methods. Chronic compression of the cervical spinal cord was implemented by implantation of a thin urethane polymer sheet under the C5-C6 laminae, which expands by absorbing tissue water over 48-72 hours. The control group underwent sham operation. Twelve weeks later, blood flow to the C3-C4 and C5-C6 spinal cord segments were measured by fluorescent microsphere methods.Results. In the control group, the blood flow in the C5-C6 segment was larger than C3-C4 segment. In the compression group, the blood flow in the C5-C6 was significantly reduced compared to the C3-C4 segment.Conclusion. Under chronic focal spinal cord compression, there was a decrease of the blood flow in the compressed segment in comparison to the rostral segment. Our data are compatible with the hypothesis that alteration in the spinal cord blood flow contributes to pathogenesis of myelopathy.