Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate.
Characterization of the Adult Head Transcriptome and Identification of Migration and Olfaction Genes in the Oriental Armyworm Mythimna separate.
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东方粘虫粘虫成虫头部转录组的表征以及迁移和嗅觉基因的鉴定
DOI：
10.1038/s41598-017-02513-6
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发表时间：
2017-05-24
影响因子：
4.6
通讯作者：
Jiang XF
中科院分区：
文献类型：
--
作者：
Bian HX;Ma HF;Zheng XX;Peng MH;Li YP;Su JF;Wang H;Li Q;Xia RX;Liu YQ;Jiang XF
粘虫是一种重要的经济昆虫，分布广泛，迁徙活动强烈。然而，关于调节东方粘虫生理和行为反应的分子机制的知识却很少。在目前的研究中，我们采取了转录转录的方法来描述分离支原体成虫头部的基因网络。测序和新组装产生63,499个转录本，进一步组装成46,459个非基因，N50为1,153个碱基。在头部转录组数据中，参与信号转导机制的Uniges最为丰富。总共有937个信号转导基因被分配给22条信号通路。然后对嗅觉基因家族的生物钟、黑色素合成和非受体蛋白进行了鉴定，并与模式昆虫Bombyxmories和其他昆虫进行了系统发育分析。此外，还鉴定了1,372个单位长度为2-6个核苷酸的简单序列重复。这些转录组数据为粘虫成虫头部提供了一个全面的分子资源，这些已鉴定的基因可以作为进一步基因功能研究的有效靶点，以解决东方粘虫迁徙和嗅觉基因调控的分子机制。
The oriental armywormMythimna separateis an economically important insect with a wide distribution and strong migratory activity. However, knowledge about the molecular mechanisms regulating the physiological and behavioural responses of the oriental armyworm is scarce. In the present study, we took a transcriptomic approach to characterize the gene network in the adult head ofM.separate. The sequencing andde novoassembly yielded 63,499 transcripts, which were further assembled into 46,459 unigenes with an N50 of 1,153 bp. In the head transcriptome data, unigenes involved in the ‘signal transduction mechanism’ are the most abundant. In total, 937 signal transduction unigenes were assigned to 22 signalling pathways. The circadian clock, melanin synthesis, and non-receptor protein of olfactory gene families were then identified, and phylogenetic analyses were performed with theseM.separategenes, the model insectBombyxmoriand other insects. Furthermore, 1,372 simple sequence repeats of 2–6 bp in unit length were identified. The transcriptome data represent a comprehensive molecular resource for the adult head ofM.separate, and these identified genes can be valid targets for further gene function research to address the molecular mechanisms regulating the migratory and olfaction genes of the oriental armyworm.