STARDUST: a pipeline for the unbiased analysis of astrocyte regional calcium dynamics.
STARDUST: a pipeline for the unbiased analysis of astrocyte regional calcium dynamics.
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STARDUST：星形胶质细胞区域钙动态公正分析的管道。
DOI：
10.1101/2024.04.04.588196
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Papouin,Thomas
中科院分区：
文献类型：
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作者：
Wu,Yifan;Dai,Yanchao;Lefton,KatherynB;Holy,TimothyE;Papouin,Thomas
钙成像已成为一种流行的方式来探测星形胶质细胞的活动，但很少有技术全面捕捉离散的钙信号发生在整个星形胶质细胞域。在这里，我们介绍STARDUST，一个管道的时空分析的区域动态和无偏排序的瞬态荧光记录的星形胶质细胞。我们描述了安装软件，检测活动像素补丁，获得活动区域（罗阿）地图，并从ROA提取时间序列的步骤。然后，我们详细介绍了使用定制的代码提取信号特征的程序。
Calcium imaging has become a popular way to probe astrocyte activity, but few techniques holistically capture discrete calcium signals occurring across the astrocyte domain. Here, we introduce STARDUST, a pipeline for the spatio-temporal analysis of regional dynamics and unbiased sorting of transients from fluorescence recordings of astrocytes. We describe steps for installing software, detecting active pixel patches, obtaining region of activity (ROA) maps, and extracting time series from ROAs. We then detail procedures for extracting signal features using custom-made code.