Purification of conjugated linoleic acid isomers through a process including lipase-catalyzed selective esterification
Purification of conjugated linoleic acid isomers through a process including lipase-catalyzed selective esterification
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DOI：
10.1271/bbb.67.1429
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发表时间：
2003-06-01
影响因子：
1.6
通讯作者：
Shimada, Y
中科院分区：
文献类型：
--
作者：
Nagao, T;Yamauchi-Sato, Y;Shimada, Y
以高纯度的亚油酸为原料，采用碱共轭法制备共轭亚油酸混合物。该制剂含有45.1重量%的顺式-9，反式-11（c9，t11）-CLA、46.8重量%的反式-10，顺式-12（t10，c12）-CLA和5.3重量%的其它CLA。一种包括Candida rugosa脂肪酶催化的选择性酯化与月桂醇，分子蒸馏，尿素加合物分馏在严格的条件下，在乙醇中的工艺是非常有效的纯化c9，t11-和t10，c12-CLA。特别地，尿素加合物分馏有效地消除了除c9，t11-和t10，c12-异构体之外的CLA。从1.0 kg CLA混合物中纯化c9，t11-和t10，c12-CLA将c9，t11-CLA纯度提高到93.1%，初始含量的回收率为34%，并将t10，c12-CLA纯度提高到95.3%，回收率为31%。
A mixture of conjugated linoleic acids (CLAs) was prepared by alkali conjugation of high purity linoleic acid. The preparation contained 45.1 wt% cis-9, trans-11 (c9,t11)-CLA, 46.8 wt% trans-10, cis-12 (t10,c12)-CLA, and 5.3 wt% other CLAs. A process comprising Candida rugosa lipase-catalyzed selective esterification with lauryl alcohol, molecular distillation, and urea adduct fractionation under strict conditions in ethanol was very effective for purification of c9,t11- and t10,c12-CLAs. In particular, the urea adduct fractionation efficiently eliminated CLAs except c9,t11- and t10,c12-isomers. Purification of c9,t11- and t10,c12-CLAs from 1.0 kg of the CLA mixture increased the c9,t11-CLA purity to 93.1% with 34% recovery of the initial content, and increased the t10,c12-CLA purity to 95.3% with 31% recovery.