In vivo bone regeneration on titanium devices using serum-free grown adipose-derived stem cells, in a sheep femur model
In vivo bone regeneration on titanium devices using serum-free grown adipose-derived stem cells, in a sheep femur model
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DOI：
10.1111/clr.12761
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发表时间：
2017-01-01
影响因子：
4.3
通讯作者：
Duncan, Warwick John
中科院分区：
文献类型：
--
作者：
Zanicotti, Diogo Godoy;Coates, Dawn Elizabeth;Duncan, Warwick John
AimThe的目的是本研究的目的是调查的能力，脂肪来源的干细胞（ADSC），生长在无血清条件下，再生骨周围的钛光盘与不同的钛surfaces.Material和methodsOvine ADSC（oADSC）分离自7只绵羊和培养使用无血清和成骨条件。在体内测试之前，使用细胞增殖和细胞外基质矿化测定法体外分析这些细胞的生长和成骨行为。在绵羊股骨上髁模型中，在钛盘上体内评价自体oADSC的骨再生能力。使用机加工（MTi）和氧化铝喷砂（ABTi）钛盘。1个月后，采用组织学和组织形态计量学评价缺损内的骨再生。PKH 26细胞示踪染料被用来验证oADSC的持久性在手术wound.ResultsoADSC来源于5的7只绵羊分化成成骨样细胞。与在ABTi和组织培养聚苯乙烯上生长的非诱导oADSC相比，仅在ABTi上生长的成骨诱导oADSC（oOS-ADSC）的细胞增殖减少（分别为P=0.03和0.02）。无论植入物表面类型如何，比较oADSC与oOS-ADSC的体外矿化试验均无显著差异。术后1个月，在缺损区检测到PKH 26标记的oADSC。与oADSC与只是blood cloak.ConclusionAfter 1个月的愈合，使用自体oADSC并没有改善骨再生的缺陷，含有不同表面的钛设备的骨缺损之间的骨再生没有差异。
AimThe aim of this study was to investigate the capacity of adipose-derived stem cells (ADSC), grown in serum-free conditions, to regenerate bone around titanium discs with different titanium surfaces.Material and methodsOvine ADSC (oADSC) were isolated from seven sheep and cultured using serum-free and osteogenic conditions. Prior to invivo testing, the growth and osteogenic behaviour of these cells were analysed invitro using cell proliferation and extracellular matrix mineralisation assays. The bone regenerative capacity of autologous oADSC was evaluated invivo on titanium discs in a sheep femur epicondyle model. Machined (MTi) and alumina-blasted (ABTi) titanium discs were used. Bone regeneration within the defects was evaluated after 1month using histology and histomorphometry. PKH26 cell-tracking dye was used to verify the persistence of oADSC in the surgical wound.ResultsoADSC sourced from five of seven sheep differentiated into osteoblast-like cells. Cellular proliferation was reduced only for osteogenically induced oADSC (oOS-ADSC) grown on ABTi, compared to non-induced oADSC grown on ABTi and tissue culture polystyrene (P=0.03 and 0.02 respectively). There was no significant difference for invitro mineralisation assays comparing oADSC with oOS-ADSC, regardless of implant surface type. oADSC labelled with PKH26 were detected 1month after surgery within the defect. There was no difference in bone regeneration between the bone defects treated with oADSC vs. just blood clot.ConclusionAfter 1-month healing, the use of autologous oADSC did not improve bone regeneration in defects containing titanium devices with different surfaces.