A Zinc Finger Protein Regulates Flowering Time and Abiotic Stress Tolerance in Chrysanthemum by Modulating Gibberellin Biosynthesis
A Zinc Finger Protein Regulates Flowering Time and Abiotic Stress Tolerance in Chrysanthemum by Modulating Gibberellin Biosynthesis
复制标题
锌指蛋白通过调节赤霉素生物合成来调节菊花的开花时间和非生物胁迫耐受性
DOI：
10.1105/tpc.114.124867
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Junping Gao
中科院分区：
文献类型：
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作者：
Yingjie Yang;Chao Ma;Yanjie Xu;Qian Wei;Muhammad Imtiaz;Lina Cheng;Haibo Lan;Zhangjun Fei;Bo Hong;Junping Gao
开花时间和对非生物胁迫的耐受能力对植物的生长发育非常重要。我们对来自菊花的锌指转录因子基因bbx24进行了鉴定，发现该基因与开花时间和抗逆性有关。抑制Cm-BBX24（Cm-BBX24- rnai）表达的转基因系比野生型植株开花时间早，对冰冻和干旱胁迫的耐受性降低。全球表达分析显示，与野生型相比，Cm-BBX24-RNAi系中与光周期和赤霉素（GA）生物合成途径相关的基因均上调。相比之下，在过表达系（Cm-BBX24-OX）中上调而在Cm-BBX24-RNAi系（均相对于野生型）中下调的基因包括与相容溶质和碳水化合物代谢相关的基因，这两个基因都与非生物胁迫有关。cm - bbx24的表达也受日长和ga4 /7的影响。在较长的日照条件下，相对于野生型，转基因系幼叶的内源GA1、GA4、GA19和ga20水平发生了变化。开花调控涉及到开花时间基因，它整合了光周期和GA生物合成途径。我们假设cm - bbx24具有双重作用，至少部分通过影响GA的生物合成来调节菊花的开花时间和非生物胁迫耐受性。
Flowering time and an ability to tolerate abiotic stresses are important for plant growth and development. We characterizedBBX24, a zinc finger transcription factor gene, fromChrysanthemum morifoliumand found it to be associated with both flowering time and stress tolerance. Transgenic lines with suppressed expression of Cm-BBX24(Cm-BBX24-RNAi) flowered earlier than wild-type plants and showed decreased tolerance to freezing and drought stresses. Global expression analysis revealed that genes associated with both photoperiod and gibberellin (GA) biosynthesis pathways were upregulated in Cm-BBX24-RNAi lines, relative to the wild type. By contrast, genes that were upregulated in overexpressing lines (Cm-BBX24-OX), but downregulated in Cm-BBX24-RNAi lines (both relative to the wild type), included genes related to compatible solutes and carbohydrate metabolism, both of which are associated with abiotic stress. Cm-BBX24expression was also influenced by daylength and GA4/7application. Under long days, changes in endogenous GA1, GA4, GA19, and GA20levels occurred in young leaves of transgenic lines, relative to the wild type. Regulation of flowering involves theFLOWERING TIMEgene, which integrates photoperiod and GA biosynthesis pathways. We postulate that Cm-BBX24plays a dual role, modulating both flowering time and abiotic stress tolerance in chrysanthemum, at least in part by influencing GA biosynthesis.