Archaeal and bacterial diversity in hot springs on the Tibetan Plateau, China
Archaeal and bacterial diversity in hot springs on the Tibetan Plateau, China
复制标题
中国青藏高原温泉中的古菌和细菌多样性
DOI：
10.1007/s00792-011-0386-z
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发表时间：
2011-09-01
期刊：
影响因子：
2.9
通讯作者：
Dong, Hailiang
中科院分区：
文献类型：
--
作者：
Huang, Qiuyuan;Dong, Christina Z.;Dong, Hailiang
采用16 SrRNA基因系统发育分析方法，对西藏中部和中东部10个海拔> 4600 m的温泉进行了古菌和细菌多样性研究。这些温泉的温度和pH值分别为26- 81 A度和接近中性。共获得959个（细菌和古细菌分别为415和544个）克隆序列。系统发育分析表明，细菌比古细菌更多样化，这些克隆序列被分为82个细菌和41个古细菌操作分类单位（OTU），分别。检索到的细菌克隆主要属于四个已知的组（即，厚壁菌门、变形菌门、蓝细菌门、绿球藻门），与低海拔地区其他中性pH温泉中的相似。相比之下，大多数来自西藏温泉的古菌克隆隶属于Thaumarchaeota，这是一个新提出的古菌门。Thaumarchaeota在西藏温泉古菌群落中的优势似乎是独一无二的，尽管确切的原因尚不清楚。统计分析表明，古菌和细菌的多样性指数与温度没有统计学相关性，这与以往的研究结果一致。
The diversity of archaea and bacteria was investigated in ten hot springs (elevation > 4600 m above sea level) in Central and Central-Eastern Tibet using 16S rRNA gene phylogenetic analysis. The temperature and pH of these hot springs were 26-81A degrees C and close to neutral, respectively. A total of 959 (415 and 544 for bacteria and archaea, respectively) clone sequences were obtained. Phylogenetic analysis showed that bacteria were more diverse than archaea and that these clone sequences were classified into 82 bacterial and 41 archaeal operational taxonomic units (OTUs), respectively. The retrieved bacterial clones were mainly affiliated with four known groups (i.e., Firmicutes, Proteobacteria, Cyanobacteria, Chloroflexi), which were similar to those in other neutral-pH hot springs at low elevations. In contrast, most of the archaeal clones from the Tibetan hot springs were affiliated with Thaumarchaeota, a newly proposed archaeal phylum. The dominance of Thaumarchaeota in the archaeal community of the Tibetan hot springs appears to be unique, although the exact reasons are not yet known. Statistical analysis showed that diversity indices of both archaea and bacteria were not statistically correlated with temperature, which is consistent with previous studies.