Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions
Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions
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DOI：
10.7554/elife.54740
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发表时间：
2020-02-18
期刊：
影响因子：
7.7
通讯作者：
Estelle, Mark
中科院分区：
文献类型：
--
作者：
Prigge, Michael J.;Platre, Matthieu;Estelle, Mark
TIR 1/AFB生长素共受体介导对植物激素生长素的不同反应。拟南芥基因组编码六个TIR 1/AFB蛋白，代表被子植物辐射前建立的四个分支中的三个。为了确定这些蛋白质在植物发育中的作用，我们进行了广泛的遗传分析，涉及所有可能的多重突变株系的产生和表征。我们发现，所有六种TIR 1/AFB蛋白的缺失导致早期胚胎缺陷并最终导致种子败育，然而TIR 1或AFB 2的单个野生型等位基因足以支持整个发育过程中的生长。我们的分析揭示了广泛的功能重叠之间，即使是最远的相关TIR 1/AFB基因，除了AFB 1。令人惊讶的是，黄曲霉毒素B1在快速生长素依赖性抑制根生长和根向地性的早期阶段具有专门的功能。这种活性可能与亚细胞定位的差异有关，与该家族的其他成员相比。
The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.