Response of the Major Allergen Fra a 1.01 in Strawberry to Cold
Response of the Major Allergen Fra a 1.01 in Strawberry to Cold
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DOI：
10.2503/hortj.utd-131
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Uno, Yuichi
中科院分区：
文献类型：
--
作者：
Okochi, Shunji;Ishibashi, Misaki;Uno, Yuichi
Fra a 1是一种草莓过敏原，会导致口腔过敏综合症。fra1.01是一种主要的异构体，在冬季大量积累在果实中。在这里，我们测试了Fra a 1.01对环境因素（如冷应激）做出反应的假设。我们分析了Fra 1.01在草莓愈伤组织和器官中的转录和翻译水平。首先，我们培养草莓（草莓x ananassa '秋姬'）愈伤组织和收获后的水果在低温下从几个小时到几天。Fra 1.01在基因表达或蛋白质积累水平上均未显示出显著差异，表明短期冷处理不影响Fra 1.01的表达。第二，我们将整株植物暴露在低温条件下约28天。在低于10 ℃的条件下，Fra 1.01转录本在整个冷处理（冠和根）或从2天到最后一天（叶和果实）逐渐诱导。28d后Fra1.01蛋白在冠部有明显积累，果实中积累较少.最后，分析Fra a 1.01e的启动子区域以检测组织特异性表达。克隆和测序的启动子包括几个顺式作用的调控元件相关的冷反应。当Fra 1.01启动子区域在拟南芥中异源表达时，通过GUS染色评估的启动子活性主要在茎尖周围和根中观察到。因此，Fra 1.01被认为在冠和根中表达，并且受到冷胁迫的加性诱导。这些器官特异性表达可能对阐明Fra 1.01蛋白在冬季果实中积累的机制具有重要意义。
Fra a 1 is a strawberry allergen that causes oral allergic syndrome. Fra a 1.01 is a major isoform that accumulates abundantly in fruits during the winter season. Here, we tested the hypothesis that Fra a 1.01 responds to environmental factors, such as cold stress. We analyzed transcriptional and translational levels of Fra a 1.01 in strawberry calli and organs under various cold conditions. First, we incubated strawberry (Fragaria x ananassa 'Akihime') calli and post-harvested fruits at low temperatures from several hours to days. Fra a 1.01 did not show significant differences in either gene expression or protein accumulation levels, suggesting that short-term cold treatments did not affect Fra a 1.01 expression. Second, we exposed whole plants to low temperature conditions for similar to 28 days. Under conditions below 10 degrees C, Fra a 1.01 transcripts were induced gradually throughout the cold treatment (crown and root), or from 2 days to the last day (leaf and fruit). The Fra a 1.01 protein remarkably accumulated in crowns and slightly in fruits after 28 days. Finally, the promoter region of Fra a 1.01e was analyzed to detect tissue-specific expression. The cloned and sequenced promoter included several cis-acting regulatory elements related to cold response. When the Fra a 1.01 promoter region was heterologously expressed in Arabidopsis, the promoter activities, as assessed by GUS staining, were observed mainly around the shoot apices and in roots. Thus, Fra a 1.01 was considered to be expressed in crowns and roots, and was additively induced by cold stress. These organ-specific expressions could be important in elucidating the mechanisms responsible for Fra a 1.01 protein's accumulation in fruits during the winter season.