Ablation of microRNAs in VIP+ interneurons impairs olfactory discrimination and decreases neural activity in the olfactory bulb
Ablation of microRNAs in VIP+ interneurons impairs olfactory discrimination and decreases neural activity in the olfactory bulb
复制标题
VIP 中间神经元中 microRNA 的消融会损害嗅觉辨别力并降低嗅球的神经活动
DOI：
10.1111/apha.13767
复制
发表时间：
2022-01-12
影响因子：
6.3
通讯作者：
Li，Anan
中科院分区：
文献类型：
--
作者：
Wu，Jing;Liu，Penglai;Li，Anan
目的微RNA（miRNAs）在表达血管活性肠肽（VIP+）的中间神经元中大量表达，并且对于它们的功能维持和存活是必不可少的。在这里，我们通过选择性地消融Dicer（一种miRNA成熟所必需的酶）来阻断小鼠有丝分裂后VIP+中间神经元中的miRNA生物合成，以研究VIP+miRNA的消融是否影响嗅觉中心（如嗅球）的嗅觉功能和神经活性，其含有大量VIP+中间神经元。使用no-go气味辨别任务和食物寻找测试来评估嗅觉辨别和嗅觉检测。在体内的电生理技术被用来记录单个单位和本地field potential.ResultsOlfactory检测和嗅觉辨别行为受损VIP+-特异性Dicer-敲除小鼠。清醒、头部固定小鼠的体内电生理记录显示，敲除小鼠二尖瓣/簇状细胞的自发和气味诱发放电率均降低。嗅球和前梨状皮质的持续和气味诱发的β局部场电位反应的功率显著降低。此外，嗅球和前梨状皮质之间的θ振荡的一致性降低。重要的是，Dicer敲除限制嗅球VIP+interneurons概括的行为和电生理结果的global knockout.ConclusionsVIP+miRNAs是一个重要的因素，在感觉加工，影响嗅觉功能和嗅觉神经活动。
AimMicroRNAs (miRNAs) are abundantly expressed in vasoactive intestinal peptide expressing (VIP+) interneurons and are indispensable for their functional maintenance and survival. Here, we blocked miRNA biogenesis in postmitotic VIP+interneurons in mice by selectively ablating Dicer, an enzyme essential for miRNA maturation, to study whether ablation of VIP+miRNA affects olfactory function and neural activity in olfactory centres such as the olfactory bulb, which contains a large number of VIP+interneurons.MethodsA go/no‐go odour discrimination task and a food‐seeking test were used to assess olfactory discrimination and olfactory detection. In vivo electrophysiological techniques were used to record single units and local field potentials.ResultsOlfactory detection and olfactory discrimination behaviours were impaired in VIP+‐specific Dicer‐knockout mice. In vivo electrophysiological recordings in awake, head‐fixed mice showed that both spontaneous and odour‐evoked firing rates were decreased in mitral/tufted cells in knockout mice. The power of ongoing and odour‐evoked beta local field potentials response of the olfactory bulb and anterior piriform cortex were dramatically decreased. Furthermore, the coherence of theta oscillations between the olfactory bulb and anterior piriform cortex was decreased. Importantly, Dicer knockout restricted to olfactory bulb VIP+interneurons recapitulated the behavioural and electrophysiological results of the global knockout.ConclusionsVIP+miRNAs are an important factor in sensory processing, affecting olfactory function and olfactory neural activity.