Mode of Subacute Sclerosing Panencephalitis (SSPE) Virus Infection in Tissue Culture Cells: III. Neurovirulence of Cell‐Free SSPE Viruses of Niigata‐1, Kitaken‐1, and Biken Strains
Mode of Subacute Sclerosing Panencephalitis (SSPE) Virus Infection in Tissue Culture Cells: III. Neurovirulence of Cell‐Free SSPE Viruses of Niigata‐1, Kitaken‐1, and Biken Strains
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组织培养细胞中亚急性硬化性全脑炎 (SSPE) 病毒感染的模式：III. Niigata-1、Kitaken-1 和 Biken 株的无细胞 SSPE 病毒的神经毒力
DOI：
10.1111/j.1348-0421.1981.tb00093.x
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发表时间：
1981
影响因子：
2.6
通讯作者：
M. Homma
中科院分区：
文献类型：
--
作者：
M. Ohuchi;R. Ohuchi;M. Homma
检查了从SSPE病毒新泻-1、Kitaken-1和Biken毒株的病毒携带培养物中回收的无细胞病毒的神经毒力。通过冻融或EDTA处理携带病毒的培养物制备无细胞病毒，并将其脑内接种至成年小鼠中。相当数量的接种小鼠在接种后约1至5周出现临床体征。首发症状为高反应性，其次为轻瘫和肌阵挛。所有受影响的小鼠都瘫痪，最后死亡。用Vero细胞与脑细胞共培养可从濒死小鼠中回收病毒。脑组织免疫荧光染色显示，含有病毒抗原的感染细胞分布稀疏。然而，就检查而言，在脑中未观察到炎症特征，并且在接种无细胞SSPE病毒的小鼠血清中未检测到抗SSPE病毒的中和抗体。
Cell‐free viruses recovered from virus‐carrying cultures of the Niigata‐1, Kitaken‐1, and Biken strains of SSPE virus were examined for neurovirulence. The cell‐free viruses were prepared by freezing and thawing or by EDTA treatment of the virus‐carrying cultures and inoculated into adult mice intracerebrally. A considerable number of the inoculated mice showed clinical signs about 1 to 5 weeks after the inoculation. The first symptom was hyperreactivity, which was followed by paresis and myoclonus. All of the affected mice fell in paralysis and finally died. The virus could be recovered from the moribund mice by cocultivation of the brain cells with Vero cells. Immunofluorescence staining of the brain tissue revealed that infected cells containing viral antigens were distributed sparsely. No inflammatory feature, however, was observed in the brain as far as examined and neutralizing antibody against SSPE virus was not detected in sera from the mice inoculated with the cell‐free SSPE viruses.