Isolation and characterization of two viruses with large genome size infecting Chrysochromulina ericina (Prymnesiophyceae) and Pyramimonas orientalis (Prasinophyceae)
Isolation and characterization of two viruses with large genome size infecting Chrysochromulina ericina (Prymnesiophyceae) and Pyramimonas orientalis (Prasinophyceae)
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DOI：
10.1006/viro.2001.1161
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发表时间：
2001-11-25
期刊：
影响因子：
3.7
通讯作者：
Bratbak, G
中科院分区：
文献类型：
--
作者：
Sandaa, RA;Heldal, M;Bratbak, G
1998年6月，从挪威沿海水域分离出两种专门针对红毛藻科（Prymnesiophyceae）和东方锥单胞菌（prinophyceae）的裂解病毒。两种病毒的裂解周期均为14 ~ 19 h；据估计，在每个宿主细胞中，黄染色体病毒的爆发大小为1800-4100个病毒，锥单胞菌病毒的爆发大小为800-1000个。感染细胞的薄片显示，两种病毒都在细胞质中复制，并且它们具有六方截面，表明二十面体对称。该病毒粒径为160 nm，基因组大小为510 kbp；主要多肽的大小为73 kDa。锥单胞菌病毒粒径为220 × 180 nm，基因组大小为560 kbp；主要多肽的大小为44 kDa。这些病毒的基因组大小是迄今为止报道过的最大的病毒之一，它们比细胞生命所需的最小值还要大。本研究描述的黄斑病毒克隆CeV-01B和锥单胞病毒克隆PoV-01B与其他感染微藻的病毒具有几个共同的特性，提示它们属于藻病毒科。(C) 2001学术出版社。
Two lytic viruses specific for Chrysochromulina ericina (Prymnesiophyceae) and for Pyramimonas orientalis (Prasinophyceae) were isolated from Norwegian coastal waters in June 1998. The lytic cycle was 14-19 h for both viruses; the burst size was estimated at 1800-4100 viruses per host cell for the Chrysochromulina virus and 800-1000 for the Pyramimonas virus. Thin sections of infected cells show that both viruses replicate in the cytoplasm and that they have a hexagonal cross section, indicating icosahedral symmetry. The Chrysochromulina virus had a particle size of 160 nm and a genome size of 510 kbp; the size of the major polypeptide was 73 kDa. The Pyramimonas virus had a particle size of 220 x 180 nm and a genome size of 560 kbp; the size of the major polypeptide was 44 kDa. The genome sizes of these viruses are among the largest ever reported for viruses and they are larger than the minimum required for cellular life. The Chrysochromulina virus clone CeV-01B and the Pyramimonas virus clone PoV-01B described in this study have several properties in common with other viruses infecting microalgae, suggesting that they belong to the Phycodnaviridae. (C) 2001 Academic Press.