The c‐Ets oncoprotein activates the stromelysin promoter through the same elements as several non‐nuclear oncoproteins.
The c‐Ets oncoprotein activates the stromelysin promoter through the same elements as several non‐nuclear oncoproteins.
复制标题
c-Ets 癌蛋白通过与几种非核癌蛋白相同的元件激活溶基质素启动子。
作者：
C. Wasylyk;A. Gutman;R. Nicholson;B. Wasylyk
c-ets原癌基因最近被证明编码转录因子，激活多瘤病毒增强子的癌基因反应单位。我们表明，在转化细胞和肿瘤中高度表达的基质分解素基因的转录通过两个DNA元件被c-Ets-1和-2有效激活。远端元件是高度保守的回文序列，由两个c-Ets-1的强结合位点组成。近端元件不结合c-Ets-1，但可能通过增加c-Jun和c-Fos的合成而间接激活。两种ets反应元件均介导癌蛋白Ha‐Ras、v‐Src和v‐Mos的激活。这些结果表明，c-Ets参与了转化细胞和肿瘤中基质分解素基因表达失调的机制。
The c‐ets protooncogenes have recently been shown to code for transcription factors that activate the oncogene responsive unit of the polyoma virus enhancer. We show that transcription of the stromelysin gene, which is highly expressed in transformed cells and tumours, is efficiently activated by c‐Ets‐1 and ‐2 through two DNA elements. The distal element is a highly conserved palindrome composed of two strong binding sites for c‐Ets‐1. The proximal element does not bind c‐Ets‐1, but may be activated indirectly by increased synthesis of c‐Jun and c‐Fos. Both ets responsive elements mediate activation by the oncoproteins Ha‐Ras, v‐Src and v‐Mos. These results suggest that c‐Ets participates in the mechanisms by which stromelysin gene expression is deregulated in transformed cells and tumours.