Local injection of substance P increases bony formation during mandibular distraction osteogenesis in rats
Local injection of substance P increases bony formation during mandibular distraction osteogenesis in rats
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局部注射 P 物质可增加大鼠下颌牵张成骨过程中的骨形成
DOI：
10.1016/j.bjoms.2014.07.002
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发表时间：
2014-10-01
影响因子：
1.8
通讯作者：
Lei, De-lin
中科院分区：
文献类型：
--
作者：
Zhang, Ya-bo;Wang, Lei;Lei, De-lin
P物质是一种神经肽，分布在支配骨髓质组织的感觉神经纤维中。它是体外成骨细胞增殖和分化的有效促进剂。然而，其促进下颌缺损修复的能力尚不清楚。我们研究了大鼠下颌骨牵张成骨过程中局部注射 P 物质的成骨作用。将20只Sprague-Dawley大鼠随机分为2组（每组10只）：实验组注射P物质10(7)mmol/l的生理盐水0.2ml，对照组注射生理盐水。下颌牵引率为每12小时0.2mm，持续10天。分散注意力期间每天注射 P 物质或盐水。使用显微计算机断层扫描 (microCT) 和组织学分析对第 15 天和第 29 天的骨再生进行定量评估。第 29 天，使用 P 物质治疗组的骨愈合率显着高于仅使用盐水组 (p = 0.001)。显微 CT 图像和定量显示，与对照组相比，给予 P 物质时有更多的愈伤组织和更成熟的皮质骨。组织学检查显示，两组牵引间隙中间均形成软骨组织。仅在最后时间点（第 29 天）物质 P 组中可见骨桥。在下颌牵张过程中，将 P 物质注射到大鼠下颌间隙中，可改善优质骨的形成并加速骨愈合。 (C) 2014 年英国口腔颌面外科医师协会。由爱思唯尔有限公司出版。保留所有权利。
Substance P is a neuropeptide that is distributed in those sensory nerve fibres that innervate the medullary tissues of bone. It is a potent accelerator of proliferation and differentiation of osteoblasts in vitro. However, its capacity for promoting repair of mandibular defects is not known. We have investigated the osteogenic effects of local injections of substance P during mandibular distraction osteogenesis in rats. Twenty Sprague-Dawley rats were randomly assigned to 2 groups (n = 10 in each): substance P 10 (7) mmol/l in normal saline 0.2 ml was injected into the experimental group, and saline alone into the controls. The mandibular distraction rate was 0.2mm every 12 hours for 10 days. Daily injections of substance P or saline were given during the distraction period. Regeneration of bone was assessed quantitatively on days 15 and 29 using microcomputed tomography (microCT), and histological analysis. The rate of bony union in the group treated with substance P was significantly higher than that in the saline alone group on day 29 (p= 0.001) The microCT images and quantitation showed more callus and more mature cortical bone when substance P was given than with control. Histological examination showed that cartilaginous tissues had formed in the middle of the distraction gaps in both groups. Bony bridges were seen only in the substance P group at the final time point (day 29). Injection of substance P into the gap of a rat mandible during mandibular distraction improved formation of good-quality bone and accelerated bony union. (C) 2014 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.