Changes in Synaptic Transmission, Calcium Current, and Neurite Growth by Perfluorinated Compounds Are Dependent on the Chain Length and Functional Group
Changes in Synaptic Transmission, Calcium Current, and Neurite Growth by Perfluorinated Compounds Are Dependent on the Chain Length and Functional Group
复制标题
全氟化合物引起的突触传递、钙电流和神经突生长的变化取决于链长和官能团。
DOI：
10.1021/es802985e
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发表时间：
2009-03-15
影响因子：
11.4
通讯作者：
Jiang, Guibin
中科院分区：
文献类型：
--
作者：
Liao, Chunyang;Wang, Thanh;Jiang, Guibin
由于全氟化合物的环境持久性、生物蓄积性和在世界各地的广泛分布，科学和公众对全氟化合物的关注日益增加。关于pfc对神经功能的影响及其潜在机制知之甚少。最近的证据表明，全氟碳化合物的毒理学效应与其碳链长度密切相关。本文研究了不同链长PFCs对培养大鼠海马神经元的作用及其可能的作用模式。在培养的神经元中，pfc灌注后自发性微型突触后电流（mPSC）频率普遍增加。mPSC频率的增加与碳链长度成正比，全氟羧酸盐的效力不如全氟磺酸盐明显。电压依赖性钙电流（I(Ca)）的幅度也有类似但不易察觉的趋势。pfc对I（Ca）激活和失活动力学的影响规律无变化。此外，长时间的pfc治疗不同程度地抑制了神经元的神经突生长。无氟和全氟类似物的比较表明，烷基链的氟化对神经元的作用比表面活性剂的活性更强。本研究表明，全氟化碳对培养的神经元有不同程度的不良影响，这取决于全氟化烷基链上的碳链长度和官能团。
Scientific and public concerns on perfluorinated compounds (PFCs) are increasingly growing because of their environmental persistency, bioaccumulation, and extensive distribution throughout the world. Little is known about the effects of PFCs on neural function and the underlying mechanisms. Recent evidence suggests that the toxicological effects of PFCs are closely correlated with their carbon chain lengths. In this present work, the actions of PFCs with varying chain length on cultured rat hippocampal neurons and possible action patterns were examined. Increases in the frequencies of spontaneous miniature postsynaptic current (mPSC) were commonly found in cultured neurons when perfused with PFCs. The increase of mPSC frequency was in proportion to the carbon chain length, and the potency of perfluorinated carboxylates was less pronounced than that of perfluorinated sulfonates. A comparable but less perceptible trend was also found for the amplitudes of voltage-dependent calcium current (I(Ca)). No regular change in pattern was observed for the effects of PFCs on activation and inactivation kinetics of I(Ca). Furthermore, prolonged treatment of PFCs inhibited the neurite growth of neuronsto various degrees. Comparisons between nonfluorinated and perfluorinated analogues demonstrated thatthefluorination in alkyl chain exerts stronger actions on neurons as compared to the surfactant activity. This study shows that PFCs exhibit adverse effects on cultured neurons to various extents, which is dependent on the carbon chain length and functional group attached to the fully fluorinated alkyl chain.