Polymorph- and Size-Dependent Uptake and Toxicity of TiO2 Nanoparticles in Living Lung Epithelial Cells
Polymorph- and Size-Dependent Uptake and Toxicity of TiO2 Nanoparticles in Living Lung Epithelial Cells
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DOI：
10.1002/smll.201001832
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发表时间：
2011-02-18
期刊：
影响因子：
13.3
通讯作者：
Osterlund, Lars
中科院分区：
文献类型：
--
作者：
Andersson, Per Ola;Lejon, Christian;Osterlund, Lars
报道了 A549 肺上皮细胞中五种类型的明确表征的锐钛矿和金红石 TiO2 纳米颗粒 (NP) 的细胞摄取和分布。静态光散射 (SLS)、体外拉曼显微光谱 (mu-Raman) 和透射电子光谱 (TEM) 揭示了纳米颗粒的内在物理化学性质、颗粒团聚和细胞纳米颗粒吸收之间的密切相关性。结果表明，mu-拉曼有利于内体颗粒细胞摄取的化学、多晶型和尺寸特异性区分以及细胞器（包括细胞核）附近颗粒的保留，这与 TEM 和 SLS 数据定量相关。深度分析 mu-拉曼结合高光谱数据分析证实了纳米颗粒在细胞中的位置，并表明纳米颗粒诱导生物基质的修饰。发现纳米粒子的吸收是动力学激活的，并且强烈依赖于硬团聚尺寸（而不是初级粒径），这在数量上与测量的细胞内氧化应激一致。还发现促炎症反应对初级粒径敏感。
The cellular uptake and distribution of five types of well-characterized anatase and rutile TiO2 nanoparticles (NPs) in A549 lung epithelial cells is reported. Static light scattering (SLS), in-vitro Raman microspectroscopy (mu-Raman) and transmission electron spectroscopy (TEM) reveal an intimate correlation between the intrinsic physicochemical properties of the NPs, particle agglomeration, and cellular NP uptake. It is shown that mu-Raman facilitates chemical-, polymorph-, and size-specific discrimination of endosomal-particle cell uptake and the retention of particles in the vicinity of organelles, including the cell nucleus, which quantitatively correlates with TEM and SLS data. Depth-profiling mu-Raman coupled with hyperspectral data analysis confirms the location of the NPs in the cells and shows that the NPs induce modifications of the biological matrix. NP uptake is found to be kinetically activated and strongly dependent on the hard agglomeration size-not the primary particle size-which quantitatively agrees with the measured intracellular oxidative stress. Pro-inflammatory responses are also found to be sensitive to primary particle size.