Cellular Response of Polyvalent Oligonucleotide-Gold Nanoparticle Conjugates
Cellular Response of Polyvalent Oligonucleotide-Gold Nanoparticle Conjugates
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DOI：
10.1021/nn102228s
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发表时间：
2010-10-01
期刊：
影响因子：
17.1
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
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作者：
Massich, Matthew D.;Giljohann, David A.;Mirkin, Chad A.
纳米颗粒在无数生物技术应用中发现了效用，包括基因调控、细胞内成像和医学诊断。因此，评价这些纳米材料的生物相容性势在必行。在这里，我们使用全基因组表达谱研究的生物反应的Hela细胞与核酸功能化的金纳米粒子。我们的研究发现，由弱结合的表面配体稳定的金纳米粒子的生物反应是显着的（细胞识别和反应的颗粒的存在），但当这些相同的纳米粒子与共价连接的核酸稳定功能化，细胞显示没有可测量的响应。这一发现对于在生物环境中研究和使用纳米材料的研究人员来说非常重要，因为它证明了表面化学和颗粒稳定性的微小变化如何导致细胞反应的显着差异。
Nanoparticles are finding utility in myriad biotechnological applications, including gene regulation, intracellular imaging, and medical diagnostics. Thus, evaluating the biocompatibility of these nanomaterials is imperative. Here we use genome-wide expression profiling to study the biological response of Hela cells to gold nanoparticles functionalized with nucleic acids. Our study finds that the biological response to gold nanoparticles stabilized by weakly bound surface ligands is significant (cells recognize and react to the presence of the particles), yet when these same nanoparticles are stably functionalized with covalently attached nucleic acids, the cell shows no measurable response. This finding is important for researchers studying and using nanomaterials in biological settings, as it demonstrates how slight changes in surface chemistry and particle stability can lead to significant differences in cellular responses.