Bradykinin-related peptides from Phyllomedusa hypochondrialis
Bradykinin-related peptides from Phyllomedusa hypochondrialis
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DOI：
10.1016/j.peptides.2006.04.020
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发表时间：
2006-09-01
期刊：
影响因子：
3
通讯作者：
Bloch, C., Jr.
中科院分区：
文献类型：
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作者：
Brand, G. D.;Krause, F. C.;Bloch, C., Jr.
采用质谱技术研究了软肋毛蚶水溶性分泌物和新鲜皮肤碎片中缓激肽相关肽（BRPs）的含量。通过从头开始的MS/MS测序和直接MALDI成像实验，对青蛙皮肤的18种BRPs及其翻译后修饰进行了鉴定。这些分子与一些哺乳动物和爬行动物的主要血浆激肽具有很强的序列相似性。这种分子的多样性，在同一个肽家族中，属于单一的两栖动物物种，可能与这些肽的功能特化和各种相应的受体有关，这些受体可能存在于许多不同的捕食者中。此外，一种新的类似物[Val](1),[Thr](6)-缓激肽gln，Ser在表达人缓激肽B-2受体的细胞培养和豚鼠回肠制剂中均有阳性的生物活性检测。(c) 2006爱思唯尔公司版权所有。
Bradykinin related peptides (BRPs) present in the water-soluble secretion and freshly dissected skin fragments of Phyllomedusa hypochondrialis were investigated by mass spectrometry techniques. Eighteen BRPs, along with their post-translational modifications, were characterized in the secretion by de novo MS/MS sequencing and direct MALDI imaging experiments of the frog skin. These molecules revealed strong sequence similarities to the main plasma kinin of some mammals and reptiles. Such a diversity of molecules, within the same peptide family, belonging to a single amphibian species may be related to functional specializations of these peptides and a variety of corresponding receptors that might be present in a number of different predators. Also, a novel analog, [Val](1),[Thr](6)-bradykinyl-Gln,Ser had its biological activity positively detected in cell culture expressing the human bradykinin B-2 receptor and in guinea pig ileum preparations. (c) 2006 Elsevier Inc. All rights reserved.