In vitro observations of self-assembled ECM-mimetic bioceramic nanoreservoir delivering rFN/CDH to modulate osteogenesis.
In vitro observations of self-assembled ECM-mimetic bioceramic nanoreservoir delivering rFN/CDH to modulate osteogenesis.
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DOI：
10.1016/j.biomaterials.2012.06.095
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发表时间：
2012-10
期刊：
影响因子：
14
通讯作者：
Y. Zhang;Liqi Li;Jie Zhu;Hong Kuang;S. Dong;Hongwei Wang;Xia Zhang;Yue Zhou
中科院分区：
文献类型：
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作者：
Y. Zhang;Liqi Li;Jie Zhu;Hong Kuang;S. Dong;Hongwei Wang;Xia Zhang;Yue Zhou
细胞-细胞外基质(ECM)相互作用已被用于指导组织特异性细胞反应和定制生物材料的开发。鉴于整合素-纤维连接蛋白相互作用和同亲钙粘连蛋白反应的重要作用，我们推测，在纳米级重组纤维连接蛋白/钙粘连蛋白嵌合体(rFN/CDH)中重建ECM的生物学功能可能会适当地解决界面上的整合障碍。本研究旨在探讨利用层层自组装技术构建rFN/CDH仿生陶瓷表面诱导成骨的可行性和有效性。X射线光电子能谱、傅里叶变换红外光谱、接触角和扫描电子显微镜证实了BCP/LBL/[CHI-rFN/CDH]多层结构的成功制备和良好的物理化学性能。在降解过程中观察到了持续的释放，多层结构还显示出显著增强的引导BMSC黏附、增殖和成骨分化的能力。机制研究表明，rFN/CDH与整合素α-5-β-1/α-v-β-3的相互作用可能是一种调控枢纽。这些结果证明了生物功能rFN/CDH多层涂层生物陶瓷在骨传导和骨诱导方面的混合潜力。因此，这项研究为制备潜在的生物功能陶瓷替代物提供了基础，用于临床前和临床应用于骨缺损和重建。
The cell-extracellular matrix (ECM) interaction has been employed to direct tissue-specific cellular responses and tailor biomaterial development. Given the important roles of integrin-fibronectin interactions and homophilic cadherin responses, we postulated that the recreation of the biological function of ECM in a recombinant fibronectin/cadherin chimera (rFN/CDH) at the nanoscale may properly address the integration impediment at the interface. This study aimed to investigate the feasibility and validity of an rFN/CDH bio-inspired ceramic surface generated via layer-by-layer (LbL) self-assembly to induce osteogenesis. The successful fabrication of a multilayered structure of BCP/LbL/[Chi-rFN/CDH] and excellent physico-chemical properties were confirmed by X-ray photoelectron spectroscopy, Fourier-transform infrared spectroscopy, contact angle, and scanning electron microscopy. Sustained release was observed during degradation, and the multilayered construct also displayed a significantly enhanced ability to guide bMSC adhesion, proliferation, and osteogenic differentiation. A mechanistic exploration revealed that the interaction of rFN/CDH with integrin α5β1/αvβ3 may be a regulatory pivot. These results demonstrate the hybrid potential of bioceramics coated with biofunctional rFN/CDH multilayers in osteoconductive and osteoinducive potency. This study therefore presents a basis for fabricating potential bio-functionalised ceramic-based substitutions for preclinical and clinical application to bone defects and reconstruction.