Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens.
Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of Salmonella enterica serovar Enteritidis for chickens.
复制标题
鸡肉肠肠肠肠肠肠肠肠球菌的五个主要致病岛（SPI-1至SPI-5）的毒力潜力用于鸡。
DOI：
10.1186/1471-2180-9-268
复制
发表时间：
2009-12-19
期刊：
影响因子：
4.2
通讯作者：
Nagy B
中科院分区：
文献类型：
--
作者：
Rychlik I;Karasova D;Sebkova A;Volf J;Sisak F;Havlickova H;Kummer V;Imre A;Szmolka A;Nagy B
沙门氏菌是一种非常成功的爬行动物、鸟类和哺乳动物寄生虫。它能够感染和定殖如此广泛的宿主，同时将新的遗传决定因素引入沙门氏菌基因组，其中包括 5 个主要致病岛 (SPI1-5)。然而，关于这些致病岛如何影响沙门氏菌感染鸡的能力，只有有限的信息。因此，在本研究中，我们构建了分别删除每个 SPI 的肠炎沙门氏菌突变体、单个 SPI（即删除了其余 4 个）和删除了所有 5 个 SPI 的突变体，并评估了它们在一日龄鸡中的毒力以及该宿主的先天免疫反应。缺乏所有 5 个主要 SPI 的突变体仍然能够定植于盲肠，而肝脏和脾脏的定植则依赖于 SPI-1 和 SPI-2 的存在。相比之下，单独缺乏 SPI-3、SPI-4 或 SPI-5 并不影响肠炎沙门氏菌对鸡的毒力，但它们共同促进了脾脏的定植。促炎信号传导和异嗜细胞浸润仅依赖于完整的 SPI-1，而不依赖于其他 SPI。 SPI-1和SPI-2是肠炎沙门氏菌在鸡全身定植所需的两个最重要的致病岛。
Salmonella is a highly successful parasite of reptiles, birds and mammals. Its ability to infect and colonise such a broad range of hosts coincided with the introduction of new genetic determinants, among them 5 major pathogenicity islands (SPI1-5), into the Salmonella genome. However, only limited information is available on how each of these pathogenicity islands influences the ability of Salmonella to infect chickens. In this study, we therefore constructed Salmonella Enteritidis mutants with each SPI deleted separately, with single individual SPIs (i.e. with the remaining four deleted) and a mutant with all 5 SPIs deleted, and assessed their virulence in one-day-old chickens, together with the innate immune response of this host. The mutant lacking all 5 major SPIs was still capable of colonising the caecum while colonisation of the liver and spleen was dependent on the presence of both SPI-1 and SPI-2. In contrast, the absence of SPI-3, SPI-4 or SPI-5 individually did not influence virulence of S. Enteritidis for chickens, but collectively they contributed to the colonisation of the spleen. Proinflammatory signalling and heterophil infiltration was dependent on intact SPI-1 only and not on other SPIs. SPI-1 and SPI-2 are the two most important pathogenicity islands of Salmonella Enteritidis required for the colonisation of systemic sites in chickens.
登录
查看更多内容
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.6
作者：
Dorsey, CW;Laarakker, MC;Bäumler, AJ
通讯作者：
Bäumler, AJ
影响因子：
3.6
作者：
Galyov, EE;Wood, MW;Wallis, TS
通讯作者：
Wallis, TS
影响因子：
2.8
作者：
Jones, Michael A.;Hulme, Scott D.;Wigley, Paul
通讯作者：
Wigley, Paul
影响因子：
3.1
作者：
Desin, Taseen S.;Lam, Po-King S.;Koester, Wolfgang
通讯作者：
Koester, Wolfgang