Reannotation of Shewanella oneidensis genome.
Reannotation of Shewanella oneidensis genome.
复制标题
DOI：
10.1089/153623103322246566
复制
发表时间：
2003-06-01
期刊：
影响因子：
--
通讯作者：
Tatusova, T.
中科院分区：
文献类型：
--
作者：
Daraselia, N.;Dernovoy, D.;Tatusova, T.
随着越来越多完整的细菌基因组序列的出现，以前测序的基因组的基因组注释可能很快就会过时。这主要是由于新基因的发现和功能表征。我们对最近发表的单氏希瓦氏菌基因组进行了重新注释，结果如下：鉴定了51个新基因，并在97个基因中添加了功能注释，其中包括15个新基因和82个已有的功能未分配的基因。新基因的识别是通过使用基于HMM的程序GeneMark.HMM预测蛋白质编码区来实现的，随后使用BLAST将预测的基因产物与非冗余蛋白质数据库进行比较，并使用COGNITOR为功能注释提供COG数据库。
As more and more complete bacterial genome sequences become available, the genome annotation of previously sequenced genomes may become quickly outdated. This is primarily due to the discovery and functional characterization of new genes. We have reannotated the recently published genome of Shewanella oneidensis with the following results: 51 new genes have been identified, and functional annotation has been added to the 97 genes, including 15 new and 82 existing ones with previously unassigned function. The identification of new genes was achieved by predicting the protein coding regions using the HMM-based program GeneMark.hmm Subsequent comparison of the predicted gene products to the non-redundant protein database using BLAST and the COG (Clusters of Orthologous Groups) database using COGNITOR provided for the functional annotation.